{
  "abstract": "Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles found in almost all body fluids. Blood-derived EVs are particularly attractive for clinical diagnostics due to their accessibility and systemic representation. However, the intrinsic complexity of EVs and the abundance of non-EV particles in blood have hindered the establishment of a standardized isolation method. Meanwhile, research on EV isolation is rapidly evolving, and there is a clear need for reviews that synthesize the recent advances in this field, particularly those focusing on blood-derived EVs. In this review, we provide a comprehensive overview of both established and emerging isolation techniques, summarizing their advantages, limitations, and applications reported over the past years, to provide methodological insights and guidance for future isolation studies.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, Liège, 4000, Belgium",
    "Corresponding author."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xi Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Jacques Crommen"
    },
    {
      "affs": [
        1
      ],
      "name": "Marianne Fillet"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3jg3ykf9scdcx.bib",
  "cited_by": 3,
  "doi": "10.1016/j.jpha.2026.101640",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S209517792600095X",
  "id": "3jg3ykf9scdcx",
  "issns": [
    "2095-1779",
    "2214-0883"
  ],
  "landing_url": "https://doi.org/10.1016/j.jpha.2026.101640",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RM1-950"
    ],
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7154749489",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/3jg3ykf9scdcx.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T14:21:05Z",
      "native_id": "0086fc7bb70d4e6085c0cee506c3644b",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.jpha.2026.101640"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Isolation of blood-derived extracellular vesicles for disease diagnosis: Methods, challenges, and applications",
  "title_text": "Isolation of blood-derived extracellular vesicles for disease diagnosis: Methods, challenges, and applications",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T14:20:18Z",
  "url": "https://research.draconitic.com/paper/3jg3ykf9scdcx",
  "venue": "Journal of Pharmaceutical Analysis",
  "versions": []
}