{
  "abstract": "Hydrogen-deuterium exchange mass spectrometry (HDX-MS) enables solution-phase characterization of protein interactions and conformational dynamics, but analysis of intact enveloped virions remains challenging because limited proteolytic accessibility restricts peptide recovery. Here, we developed an acoustic-assisted HDX-MS workflow integrating chemical denaturation with bath sonication during quenching and applied it to dengue virus serotype 2 (DENV2) strain D2Y98P. The workflow achieved sequence coverages of 96.0 ± 0.7%, 89.8 ± 0.8% and 87.1 ± 1.0% for the capsid (C), envelope (E) and membrane (M) proteins, respectively. Model peptide measurements showed comparable back-exchange between sonication and incubation at matched durations, although longer treatment increased deuterium loss. Improved E protein coverage enabled characterization of changes in deuterium uptake associated with binding of the neutralizing antibody 2D22. Protected regions overlapped spatially with the cryo-EM-defined quaternary interface, while additional protection occurred in regions remote from direct antibody contact. These findings are consistent with antibody-associated conformational stabilization extending beyond the binding interface. Acoustic-assisted HDX-MS therefore improves structural protein coverage and complements cryo-EM by revealing peptide-level changes in antibodyassociated protein dynamics in the intact virion context.",
  "abstract_shown": true,
  "affiliations": [
    "School of Biological Sciences",
    "Nanyang Technological University",
    "School of Chinese Materia Medica & Key Laboratory of Chinese Internal Medicine",
    "Beijing University of Chinese Medicine"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Li Ching Pang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ying Deng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Vinayak Gupta"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Wen Zheng Lo"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Shuangshuang Zhu"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Muen Shan"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Marrisa, Mei Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xueming Dong",
      "orcid": "0000-0001-8726-7565"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3jxrm13fdz5k2.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15009664/v1",
  "fulltext_url": null,
  "id": "3jxrm13fdz5k2",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009664/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Mass Spectrometry Techniques and Applications"
    ]
  ],
  "openalex": "W7214953253",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15009664/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-29",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/3jxrm13fdz5k2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:21Z",
      "native_id": "10.26434/chemrxiv.15009664",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009664/v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Mass Spectrometry Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Acoustic-assisted HDX-MS for Intact Virion Analysis",
  "title_text": "Acoustic-assisted HDX-MS for Intact Virion Analysis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:21Z",
  "url": "https://research.draconitic.com/paper/3jxrm13fdz5k2",
  "venue": "ChemRxiv",
  "versions": []
}