{
  "abstract": "Diplonemids are highly abundant unicellular flagellates that are evolutionarily divergent from traditional model eukaryotes. Diplonema papillatum is a model diplonemid for which several molecular tools have recently been established. However, no method for conditional protein depletion was available. Here, we have adapted an auxin-inducible degron (AID) system in D. papillatum. Depletion of the KIN-A motor protein (a glycomonad-specific component of the chromosomal passenger complex) causes defects in chromosome segregation and cell proliferation. The AID system also successfully depleted a divergent Mad1 protein that localizes at nuclear pores, and a flagellar component, CFAP20. Our study demonstrates the power of the AID system to perform loss-of-function studies in D. papillatum.",
  "abstract_shown": true,
  "affiliations": [
    "University of Edinburgh"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "G. Clifford"
    },
    {
      "affs": [
        0
      ],
      "name": "B. Akiyoshi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3k88zfj1q13r5.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.11.751042",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.11.751042v1.full",
  "id": "3k88zfj1q13r5",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.11.751042v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1307|Microtubule and mitosis dynamics"
    ]
  ],
  "openalex": "W7212700185",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.11.751042v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-11",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3k88zfj1q13r5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:13Z",
      "native_id": "10.64898/2026.09.11.751042",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.11.751042v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Auxin-inducible degron-mediated protein depletion in Diplonema papillatum",
  "title_text": "Auxin-inducible degron-mediated protein depletion in Diplonema papillatum",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:13Z",
  "url": "https://research.draconitic.com/paper/3k88zfj1q13r5",
  "venue": "bioRxiv",
  "versions": [
    {
      "doi": "10.12688/wellcomeopenres.27732.1",
      "pid": null,
      "relation": "published-as",
      "title": null,
      "venue": null
    }
  ]
}