{
  "abstract": "Constructing human vascularized artificial tissues (HVATs) that form stable vascular structures after transplantation remains challenging in regenerative medicine. Using the cryopreserved cell accumulation method (CP-CAM), a scaffold-free approach based on extracellular matrix nanofilm-coated cells, we generated HVATs from human adipose tissue-derived mesenchymal stromal cells (hASCs) and cord blood- or peripheral blood-derived endothelial colony-forming cells (hCB-ECFCs and hPB-ECFCs). Both ECFCs formed vascular networks within four days after seeding, with no significant differences in two-dimensional vascular length density or area fraction compared with HUVEC-based constructs. hCB-ECFC-derived vascular structures showed incomplete endothelial closure and reduced perivascular differentiation of hASCs in vitro, whereas hPB-ECFC-derived structures exhibited more continuous vascular morphology. Angiogenesis-related factor profiling of HVAT supernatants suggested distinct patterns among endothelial cell sources. After subcutaneous transplantation into nude mice, hCB-ECFC-derived vessels exhibited continuous endothelial lumens and surrounding pericyte- or smooth muscle-like cells, with further structural maturation over four weeks. HVATs constructed with hPB-ECFCs also engrafted and formed human-derived vascular structures containing host blood cells and surrounded by perivascular cells. These findings demonstrate that both hCB-ECFCs and hPB-ECFCs can be used to construct transplantable HVATs by CP-CAM and support the potential of blood-derived ECFCs as endothelial cell sources for vascularized tissue engineering and therapeutic applications.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Yoshiya Asano"
    },
    {
      "name": "Yoshiko Tamai"
    },
    {
      "name": "Hiroshi Shimoda"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3q054hvk0pvtr.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-73908-7",
  "fulltext_url": null,
  "id": "3q054hvk0pvtr",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-73908-7",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2746|Tissue Engineering and Regenerative Medicine"
    ]
  ],
  "openalex": "W7214901069",
  "pdf_url": "https://www.nature.com/articles/s41598-026-73908-7_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/3q054hvk0pvtr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:19Z",
      "native_id": "10.1038/s41598-026-73908-7",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-73908-7"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2746|Tissue Engineering and Regenerative Medicine",
      "subfield": "surgery"
    }
  ],
  "title": "In vivo engraftment of vascular structures in 3D-engineered artificial tissues vascularized by cord blood- or peripheral blood-derived endothelial colony-forming cells",
  "title_text": "In vivo engraftment of vascular structures in 3D-engineered artificial tissues vascularized by cord blood- or peripheral blood-derived endothelial colony-forming cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:19Z",
  "url": "https://research.draconitic.com/paper/3q054hvk0pvtr",
  "venue": "Scientific Reports",
  "versions": []
}