{
  "abstract": "Astrovirus, cardiovirus and rotavirus are RNA viruses widely distributed among humans, domestic and wild mammals, including felids, occasionally associated with fatal outbreaks and highly resistant to environmental conditions. Despite this, there are no reports of these viruses in New World felids. The aim of this study was to investigate the presence of astrovirus, rotavirus and cardiovirus in non-domestic felids under human care or in the wild, across multiple Brazilian biomes (i.e., Amazon Forest, Atlantic Forest, Caatinga, and Cerrado). Biological samples (e.g., blood, oral and rectal swabs, and frozen tissues) were submitted to reverse transcriptase polymerase chain reaction (RT-PCR) for astrovirus detection and real-time RT-PCR for rotavirus and cardiovirus, followed by sequencing. Fifty wild felids were analyzed, comprising 40 free-ranging and 10 captive animals: 19 ocelots (Leopardus pardalis), 12 pumas (Puma concolor), seven jaguarundis (Herpailurus yagouaroundi), six southern tiger cats (Leopardus guttulus), three margays (Leopardus wiedii) and three jaguars (Panthera onca). An astrovirus was detected in rectal swab of a healthy captive southern tiger cat, while a genotype G3P[3] Rotavirus alphagastroenteritidis (Rotavirus A) was detected in intestine of a roadkill puma. All felid samples were negative for cardiovirus. Morphological analyses of available formalin-fixed tissues of the rotavirus-positive puma were inconclusive due to autolysis. To the authors’ knowledge, this is the first molecular detection of astrovirus and rotavirus in Neotropical wild felid species.",
  "abstract_shown": true,
  "affiliations": [
    "Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Júlio de Mesquita Filho, Botucatu, São Paulo, Brazil",
    "Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil",
    "Grupo de Epidemiología y Sanidad Ambiental, Centro de Investigación en Sanidad Animal, INIA-CSIC, Madrid, Spain",
    "Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil",
    "Instituto Baleia Jubarte, Bahia, Brazil",
    "Panthera, New York, New York, United States of America",
    "Centro Nacional de Pesquisa e Conservação de Mamíferos Carnívoros, Atibaia, São Paulo, Brazil",
    "Zoológico Municipal Quinzinho de Barros, Sorocaba, São Paulo, Brazil",
    "Faculdade de Medicina Veterinária, Universidade de Sorocaba, Sorocaba, São Paulo, Brazil"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Michelle Colpani"
    },
    {
      "affs": [
        1
      ],
      "name": "Aricia Duarte-Benvenuto"
    },
    {
      "affs": [
        2
      ],
      "name": "Carlos Sacristán"
    },
    {
      "affs": [
        3
      ],
      "name": "Marina Galvão Bueno"
    },
    {
      "affs": [
        4
      ],
      "name": "Marcia Terezinha Baroni de Moraes"
    },
    {
      "affs": [
        5
      ],
      "name": "Roberta Zamana-Ramblas"
    },
    {
      "affs": [
        6
      ],
      "name": "Bárbara Sophia Codeas"
    },
    {
      "affs": [
        7
      ],
      "name": "Pedro Enrique Navas-Suarez"
    },
    {
      "affs": [
        8
      ],
      "name": "María Alejandra Arias-Lugo"
    },
    {
      "name": "Ronaldo Gonçalves Morato"
    },
    {
      "name": "Rose Lilian Gasparini-Morato"
    },
    {
      "name": "Rodrigo Hidalgo Friciello Teixeira"
    },
    {
      "name": "Sheila Canevese Rahal"
    },
    {
      "name": "José Luiz Catão-Dias"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3sgxrex1pw5r5.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0359169",
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  "id": "3sgxrex1pw5r5",
  "issns": [
    "1932-6203"
  ],
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  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "openalex",
      "2725|Viral gastroenteritis research and epidemiology"
    ],
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      "plos",
      "/Biology and life sciences/Anatomy/Digestive system/Gastrointestinal tract/Rectum"
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      "/Biology and life sciences/Zoology/Animals/Vertebrates/Amniotes/Mammals/Cats/Pumas"
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      "/Medicine and health sciences/Anatomy/Digestive system/Gastrointestinal tract/Rectum"
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      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Viral pathogens/Astroviruses"
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      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Viral pathogens/Reoviruses/Rotavirus"
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  "pmcid": null,
  "pmid": null,
  "published": "2026-10-16",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/3sgxrex1pw5r5.ris",
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    {
      "first_seen": "2026-10-30T12:17:23Z",
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  "subjects": [
    {
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      "subfield": "microbiology"
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  "title": "Molecular screening of selected RNA viruses in neotropical wild felids of Brazil",
  "title_text": "Molecular screening of selected RNA viruses in neotropical wild felids of Brazil",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:23Z",
  "url": "https://research.draconitic.com/paper/3sgxrex1pw5r5",
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