{
  "abstract": "Target leaf spot (TLS), caused by Corynespora cassiicola, is among the most economically important diseases of cucumber (Cucumis sativus L.). Because of the rising incidence of disease problems, much effort has been put toward resistance breeding. Here, we investigated the inheritance of high TLS resistance in a Japanese-type inbred line. QTL analyses were performed using F₂ and F2:3 families derived from a cross between the resistant and a susceptible (Beit Alpha-type) inbred line. We evaluated disease severity in F2:3 families in four independent assays and mapped a total of five TLS-resistance QTLs on chromosome (Chr.) 2, Chr. 5, and Chr. 6. The effect of the two major QTLs on Chr.5, Tls5.1 and Tls5.2, was confirmed through additional assays and verified in different genetic backgrounds. Fine mapping using recombinants in several generations narrowed down Tls5.2 to a 496.8-kb region with 69 genes, several of which are potential candidates involved in plant disease resistance. The newly identified resistance loci may confer more durable resistance when combined with previously characterized resistance genes or loci. Furthermore, molecular markers linked to TLS resistance derived from the Japanese-type inbred line could be effectively used to accelerate breeding for this trait.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.",
    "Institute of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.",
    "Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Minh Thi Ho"
    },
    {
      "affs": [
        1
      ],
      "name": "Ruikun Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Sachiko Isobe"
    },
    {
      "affs": [
        2
      ],
      "name": "Kenta Shirasawa"
    },
    {
      "affs": [
        1
      ],
      "name": "Yosuke Yoshioka"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3ssf8h4060ahg.bib",
  "cited_by": 0,
  "doi": "10.1270/jsbbs.26005",
  "fulltext_url": "https://europepmc.org/articles/PMC13709615",
  "id": "3ssf8h4060ahg",
  "issns": [
    "1344-7610",
    "1347-3735"
  ],
  "landing_url": "https://europepmc.org/article/MED/42936844",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Advances in Cucurbitaceae Research"
    ]
  ],
  "openalex": "W7166864969",
  "pdf_url": "https://europepmc.org/articles/PMC13709615?pdf=render",
  "pmcid": "PMC13709615",
  "pmid": "42936844",
  "published": "2026-06-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3ssf8h4060ahg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42936844",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42936844"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Advances in Cucurbitaceae Research",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "QTL mapping for target leaf spot resistance in a Japanese-type cucumber.",
  "title_text": "QTL mapping for target leaf spot resistance in a Japanese-type cucumber.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/3ssf8h4060ahg",
  "venue": "Breeding science",
  "versions": []
}