{
  "abstract": "Head and neck squamous cell carcinoma (HNSCC) develops resistance to cisplatin. We investigated whether JIB-04, an inhibitor of Jumonji histone demethylases, enhances cisplatin activity in established HNSCC cell lines. Four UT-SCC cell lines originating from primary tumors and matched lymph-node metastases were treated with JIB-04 and cisplatin, alone or combined. Viability was assessed by MTT assay, and drug interactions were evaluated using the Highest Single Agent model. In UT-SCC-24A cells, we assessed BrdU incorporation, LDH release, cell-cycle distribution, and apoptosis. RNA sequencing was performed after 24 h at the same drug concentrations used in functional assays. JIB-04 reduced viability in all HNSCC lines at submicromolar concentrations, with moderate effects in BJ fibroblasts. HSA analysis identified a localized positive interaction in UT-SCC-24A, where the overall HSA score was 4.25, whereas local scores reached approximately 16–18 within a narrow low-dose range. Co-treatment further reduced DNA synthesis and increased S/G2-M accumulation and apoptosis, without significantly increasing LDH release. RNA sequencing revealed JIB-04-associated induction of integrated stress response/ATF4- and DNA damage response-related genes, alongside suppression of proliferation- and cell-cycle-associated genes. The combination retained these transcriptional features. These findings support the further investigation of JIB-04–cisplatin combinations in selected HNSCC cell-line models.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland",
    "Department of Physiology and Toxicology, The John Paul II Catholic University of Lublin, 20-708 Lublin, Poland"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kamila Adamczuk"
    },
    {
      "affs": [
        1
      ],
      "name": "Grzegorz Adamczuk",
      "orcid": "0000-0003-1677-7247"
    },
    {
      "affs": [
        0
      ],
      "name": "Michał Kiełbus",
      "orcid": "0000-0001-6156-6217"
    },
    {
      "affs": [
        0
      ],
      "name": "Matthias Nees",
      "orcid": "0000-0002-9034-301X"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrzej Stepulak",
      "orcid": "0000-0002-1872-394X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3te78d9bap1x8.bib",
  "cited_by": 0,
  "doi": "10.3390/cells15212023",
  "fulltext_url": null,
  "id": "3te78d9bap1x8",
  "issns": [
    "2073-4409"
  ],
  "landing_url": "https://www.mdpi.com/2073-4409/15/21/2023",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/3te78d9bap1x8.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T12:21:10Z",
      "native_id": "10.3390/cells15212023",
      "source": "crossref",
      "url": "https://www.mdpi.com/2073-4409/15/21/2023"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:2073-4409",
      "subfield": "cell-biology"
    }
  ],
  "title": "Enhancement of Cisplatin Efficacy by the Histone Demethylase Inhibitor JIB-04 in Head and Neck Squamous Cell Carcinoma (HNSCC) Cell Lines",
  "title_text": "Enhancement of Cisplatin Efficacy by the Histone Demethylase Inhibitor JIB-04 in Head and Neck Squamous Cell Carcinoma (HNSCC) Cell Lines",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T00:22:31Z",
  "url": "https://research.draconitic.com/paper/3te78d9bap1x8",
  "venue": "Cells",
  "versions": []
}