{
  "abstract": "Numerous studies have reported Up-frameshift protein 1 (UPF1) as a crucial posttranscriptional regulator in nonsense-mediated mRNA decay (NMD), attributing its role to tumorigenesis across various cancers. However, its recently unveiled function in UPF1-mediated mRNA decay (UMD), intricately coordinated with miRNA, remains unexplored in the context of tumorigenesis. Here, we demonstrate that UPF1 contributes to melanoma tumorigenesis by regulating NANOS1 expression in a miR-101-3p-dependent manner. Depletion of UPF1 across three melanoma cell lines significantly impaired cell growth, migration, and invasion by cell cycle arrest and disrupted tumor formation in vivo. Through comprehensive RNA-seq and biochemical analyses, we elucidate that attenuation of UPF1 leads to an increase in NANOS1 expression, consequently suppressing melanoma tumorigenesis. Mechanistically, UPF1 orchestrates the destabilization of NANOS1 via UPF1-mediated mRNA decay (UMD) facilitated by miR-101-3p. Intriguingly, UPF1 depletion and NANOS1 upregulation were associated with reduced ERK and ELK1 phosphorylation, suggesting that attenuation of MAPK/ERK/ELK1 signaling may contribute, at least in part, to the observed melanoma-suppressive phenotypes. Importantly, the biological relevance of UPF1 and NANOS1 in melanoma cell lines was further supported in drug-resistant melanoma cells and in xenograft mouse models. Collectively, these findings expand our understanding of UMD in melanoma and identify the UPF1/NANOS1 regulatory relationship as a potential therapeutic vulnerability in both parental and drug-resistant melanoma cells.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School for Biomedical Science & Engineering, Hanyang University, Seoul, Korea.",
    "Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea.",
    "Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, Korea.",
    "Research Institute for Natural Sciences, Hanyang University, Seoul, Republic of Korea.",
    "Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, Republic of Korea."
  ],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Suman Lee"
    },
    {
      "affs": [
        1
      ],
      "name": "Jae-Won Yoon"
    },
    {
      "affs": [
        0
      ],
      "name": "Dayeon Woo"
    },
    {
      "affs": [
        0
      ],
      "name": "Hyunho Cha"
    },
    {
      "affs": [
        0
      ],
      "name": "Seungwon Jung"
    },
    {
      "affs": [
        1,
        2,
        3,
        4
      ],
      "name": "Je-Min Choi"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Jungwook Hwang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3v07ehfbzdaqd.bib",
  "cited_by": 0,
  "doi": "10.7150/ijbs.134804",
  "fulltext_url": "https://europepmc.org/articles/PMC13685752",
  "id": "3v07ehfbzdaqd",
  "issns": [
    "1449-2288"
  ],
  "landing_url": "https://europepmc.org/article/MED/42909927",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|MicroRNA in disease regulation"
    ]
  ],
  "openalex": "W7214602023",
  "pdf_url": "https://europepmc.org/articles/PMC13685752?pdf=render",
  "pmcid": "PMC13685752",
  "pmid": "42909927",
  "published": "2026-09-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3v07ehfbzdaqd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:14Z",
      "native_id": "MED:42909927",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42909927"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|MicroRNA in disease regulation",
      "subfield": "oncology"
    }
  ],
  "title": "UPF1-mediated mRNA decay promotes melanoma tumorigenesis through miR-101-3p-dependent destabilization of NANOS1.",
  "title_text": "UPF1-mediated mRNA decay promotes melanoma tumorigenesis through miR-101-3p-dependent destabilization of NANOS1.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:14Z",
  "url": "https://research.draconitic.com/paper/3v07ehfbzdaqd",
  "venue": "International journal of biological sciences",
  "versions": []
}