{
  "abstract": "Subcellular RNA localization serves as a critical regulatory layer governing cellular polarization, local translation, and functional compartmentalization. Recent advances in high-resolution spatial transcriptomics have provided unprecedented opportunities to resolve these intracellular architectures at single-molecule resolution. However, relevant datasets are dispersed across studies and repositories, vary substantially in processing procedures and standards, severely limiting their cross-study reuse. To bridge this gap, we present STRAND (Subcellular-Resolved Spatial Transcriptome RNA Architecture and Navigation Database), a curated data resource and analysis platform for the automated harvesting, quality control, and interpretation of subcellular spatial transcriptomics data. Through an agent-assisted curation pipeline, STRAND has assembled 35 subcellular-resolution datasets from six major platforms, covering 17 tissue types in human and mouse and totalling ∼4.9 billion transcript coordinates. Building upon this standardized data resource, STRAND provides an analytical workflow spanning cell segmentation evaluation, cell-type annotation, RNA localization/colocalization pattern annotation, and subcellular compartment inference. Two case studies demonstrate that STRAND successfully identifies established RNA localization patterns in cultured cell lines and detects drug-induced RNA relocalization in cardiomyocytes. STRAND is openly available at https://strand.phasep.pro.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing 100191 ,",
    "Key Laboratory for Neuroscience, Ministry of Education/National Health Commission of China, Peking University , Beijing 100191 ,",
    "Institute of Biotechnology Innovation, Peking University , Beijing 100871 ,",
    "School of Software Engineering, Beijing Jiaotong University , Beijing 100044 ,",
    "State Key Laboratory of Natural and Biomimetic Drugs, Peking University , Beijing 100191 ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhaoxu Wu"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Yuying Huo"
    },
    {
      "affs": [
        3
      ],
      "name": "Wenqian Xu"
    },
    {
      "affs": [
        3
      ],
      "name": "Xiangyu Li",
      "orcid": "0000-0002-6017-0701"
    },
    {
      "affs": [
        0,
        1,
        4
      ],
      "name": "Tingting Li",
      "orcid": "0000-0003-4266-0317"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3w35dy329y7zz.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag1054",
  "fulltext_url": null,
  "id": "3w35dy329y7zz",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkag1054/9005085",
  "language": null,
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": "https://academic.oup.com/nar/advance-article-pdf/doi/10.1093/nar/gkag1054/72614291/gkag1054.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/3w35dy329y7zz.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:50Z",
      "native_id": "10.1093/nar/gkag1054",
      "source": "crossref",
      "url": "https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkag1054/9005085"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:0305-1048",
      "subfield": "molecular-biology"
    }
  ],
  "title": "STRAND: a comprehensive subcellular-resolved spatial transcriptome RNA architecture and navigation database",
  "title_text": "STRAND: a comprehensive subcellular-resolved spatial transcriptome RNA architecture and navigation database",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T00:20:50Z",
  "url": "https://research.draconitic.com/paper/3w35dy329y7zz",
  "venue": "Nucleic Acids Research",
  "versions": []
}