{
  "abstract": "Arsenic trioxide (ATO) is a potent therapeutic agent against acute promyelocytic leukemia; nevertheless, its clinical utility is severely restricted by dose-limiting nephrotoxicity. This study investigated the protective potential of a radiation-synthesized gallotannin hydrogel (GTH) against ATO-induced kidney injury in rats. Forty male Wistar rats were randomized into four experimental groups: Control, ATO (4 mg/kg, i.p., for 4 weeks), GTH (2 mg/kg, i.p.), and ATO + GTH. GTH treatment was initiated during the third week of ATO administration, with GTH being administrated one hour prior to ATO injection. Following the experimental period, animals were sacrificed, tissue histology was examined, and renal function was assessed by serum urea and creatinine levels. Oxidative stress was evaluated via MDA, SOD, GSH, and GPx markers. Additionally, miR-223 and TXNIP expression levels were quantified using qRT-PCR. Inflammatory mediators (NF-κB, NLRP3, Caspase-1, IL-1β, and IL-18), endoplasmic reticulum (ER) stress markers (PERK, eIF2α, and CHOP), and the apoptotic marker caspase-3 were analyzed by ELISA. Our results revealed that GTH noticeably ameliorated kidney dysfunction, as evidenced by reduced serum urea and creatinine levels. Furthermore, GTH attenuated oxidative stress by decreasing MDA levels and restoring antioxidant enzyme activities (SOD, GSH, and GPx). Histological analysis confirmed that GTH mitigated structural renal damage. Molecularly, GTH administration suppressed the ATO-induced upregulation of miR-223 and TXNIP. This downregulation correlated with a reduction in inflammatory mediators, attenuation of ER stress markers, and decreased apoptosis. These findings demonstrate that radiation-synthesized gallotannin hydrogel (GTH) exerts nephroprotective effects against ATO-induced nephrotoxicity. These benefits are driven by antioxidant, anti-inflammatory, and anti-apoptotic mechanisms mediated via modulation of the miR-223/TXNIP axis.\n\nGraphical Abstract:",
  "abstract_shown": true,
  "affiliations": [
    "Biochemistry and Molecular Biology Department, Faculty of veterinary Medicine, Benha University, Benha, Egypt",
    "Radiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt",
    "Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Omayma A.R. Abo-Zaid",
      "orcid": "0000-0002-1752-6709"
    },
    {
      "affs": [
        1
      ],
      "name": "Mostafa A. Farrag",
      "orcid": "0000-0001-5829-6260"
    },
    {
      "affs": [
        2
      ],
      "name": "Aya S. R. Shaaban",
      "orcid": "0000-0002-6479-196X"
    },
    {
      "affs": [
        2
      ],
      "name": "Hiam Rushdy A Salem",
      "orcid": "0000-0001-6648-0404"
    },
    {
      "affs": [
        1
      ],
      "name": "Marwa A. Esmat",
      "orcid": "0000-0002-4608-4540"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3w52a02ffjqbt.bib",
  "cited_by": 0,
  "doi": "10.1007/s12011-026-05301-4",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13714069/",
  "id": "3w52a02ffjqbt",
  "issns": [
    "0163-4984",
    "1559-0720"
  ],
  "landing_url": "https://doi.org/10.1007/s12011-026-05301-4",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "2734|Chemotherapy-induced organ toxicity mitigation"
    ]
  ],
  "openalex": "W7207575471",
  "pdf_url": "https://link.springer.com/content/pdf/10.1007/s12011-026-05301-4.pdf",
  "pmcid": "PMC13714069",
  "pmid": "42693366",
  "published": "2026-09-04",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3w52a02ffjqbt.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:21:38Z",
      "native_id": "PMC13714069",
      "source": "pmc",
      "url": "https://doi.org/10.1007/s12011-026-05301-4"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2734|Chemotherapy-induced organ toxicity mitigation",
      "subfield": "general"
    }
  ],
  "title": "“Radiation-Synthesized Gallotannin Hydrogel Attenuates Trisenox-Induced Nephrotoxicity in Rats: miR-223/TXNIP Axis Modulation of Oxidative Stress and Inflammation”",
  "title_text": "“Radiation-Synthesized Gallotannin Hydrogel Attenuates Trisenox-Induced Nephrotoxicity in Rats: miR-223/TXNIP Axis Modulation of Oxidative Stress and Inflammation”",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:38Z",
  "url": "https://research.draconitic.com/paper/3w52a02ffjqbt",
  "venue": "Biological Trace Element Research",
  "versions": []
}