{
  "abstract": "Background\n\nTobacco bacterial wilt, a devastating soil-borne disease caused by the Ralstonia solanacearum species complex (RSSC), poses a severe threat to tobacco production.Results\n\nIn this study, a predominant Ralstonia solanacearum strain, designated R1, was isolated from a typical diseased tobacco field in Zhangjiajie, Hunan Province, China. The strain was characterized through a combination of morphological observation, growth curve analysis, and phenotypic profiling using Biolog GEN III MicroPlates. These assays collectively revealed its key morphological features and physiological and biochemical properties. Whole-genome sequencing revealed that the R1 genome is 4.92 Mb in size, with a GC content of 66.80%, comprising a circular chromosome and a 1.03-Mb megaplasmid. Comparative genomic and phylogenomic analyses further assigned strain R1 to Phylotype I, which is characteristic of Asian-origin strains. Genome analysis identified 400 potential virulence factors, 233 antibiotic resistance genes (ARGs), and 7 predicted biosynthetic gene clusters (BGCs) for secondary metabolites. Notably, two non-ribosomal peptide synthetase (NRPS)-related clusters exhibited high homology to known gene clusters responsible for synthesizing the antimicrobial compounds micacocidin and rhizomide.Conclusions\n\nThis study presents the first integrated genomic and phenotypic analysis of an R. solanacearum strain from the Zhangjiajie ecological region in western Hunan. The findings provide key insights into the adaptive evolution and pathogenic mechanisms of the local pathogen, as well as crucial theoretical foundations and genetic targets for breeding region-specific, disease-resistant tobacco varieties, novel green control strategies based on quorum-sensing interference, and science-based guidelines for antibiotic use in agriculture.",
  "abstract_shown": true,
  "affiliations": [
    "Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, Yunnan, 650224, China.",
    "Jiangxi Provincial Key Laboratory of Plantation and High Valued Utilization of Specialty Fruit Tree and Tea, Institute of Resources and Environment, Jiangxi Academy of Sciences, Nanchang, Jiangxi, 330096, China.",
    "College of Land and Environment, Shenyang Agictlhral University, Shenyang, Liaoning, 110866, China.",
    "Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China. liuyi@isa.ac.cn.",
    "Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, Yunnan, 650224, China. zhangjunzhong@swfu.edu.cn.",
    "Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, Yunnan, 650224, China. zhangjunzhong@swfu.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yanmei He"
    },
    {
      "affs": [
        1
      ],
      "name": "Dongliang Yao"
    },
    {
      "affs": [
        2
      ],
      "name": "Shipei Zu"
    },
    {
      "affs": [
        3
      ],
      "name": "Yi Liu"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Junzhong Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3wd0xcnakgj8a.bib",
  "cited_by": 0,
  "doi": "10.1186/s12870-026-09505-7",
  "fulltext_url": "https://europepmc.org/articles/PMC13700292",
  "id": "3wd0xcnakgj8a",
  "issns": [
    "1471-2229"
  ],
  "landing_url": "https://europepmc.org/article/MED/42928122",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Pathogenic Bacteria Studies"
    ]
  ],
  "openalex": "W7171488736",
  "pdf_url": "https://europepmc.org/articles/PMC13700292?pdf=render",
  "pmcid": "PMC13700292",
  "pmid": "42928122",
  "published": "2026-07-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3wd0xcnakgj8a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:20Z",
      "native_id": "MED:42928122",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42928122"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Pathogenic Bacteria Studies",
      "subfield": "plant-biology"
    }
  ],
  "title": "Integrated phenotypic and genome-wide characterization of Ralstonia solanacearum strain R1 of tobacco.",
  "title_text": "Integrated phenotypic and genome-wide characterization of Ralstonia solanacearum strain R1 of tobacco.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:20Z",
  "url": "https://research.draconitic.com/paper/3wd0xcnakgj8a",
  "venue": "BMC plant biology",
  "versions": []
}