{
  "abstract": "Foot-and-mouth disease (FMD) remains a major burden in endemic regions, where inactivated vaccines are constrained by cost, short duration of immunity, cold-chain dependence and high-containment manufacturing. Here, we engineered a recombinant bovine papular stomatitis virus (rBPSV) expressing the FMD virus (FMDV) A24 Cruzeiro capsid precursor P1-2A together with an attenuated 3C protease (3CproL127P). Infection of ovine foetal turbinate cells resulted in FMDV capsid protein expression and assembly of 25-30 nm icosahedral virus-like particles (VLPs). Intramuscular immunization of two BPSV-seropositive calves with rBPSV (10⁷ TCID₅₀ on days 0, 21 and 35) induced anti-FMDV humoral responses. By day 28, liquid-phase blocking ELISA titres exceeded laboratory-defined protective threshold (≥1.9 log₁₀), and homologous neutralizing titres against A24 Cruzeiro surpassed the threshold associated with protection (≥1.36 log₁₀). Intra-serotypic cross-neutralization was observed against A/Argentina/2001, whereas no neutralization was detected against heterologous serotype O₁ Campos. Pre-existing anti-BPSV antibodies did not prevent induction of neutralizing responses. These findings establish the first proof of concept that BPSV can serve as a cattle-adapted vector platform for delivery of FMDV VLPs.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA",
    "School of Veterinary Medicine and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA",
    "Instituto de Virología e Innovaciones Tecnológicas, CICVyA, INTA-CONICET, Buenos Aires, Argentina",
    "WOAH FMD Reference Laboratory, SENASA, Buenos Aires, Argentina"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Herman M. Chambaro",
      "orcid": "0000-0003-1407-6818"
    },
    {
      "affs": [
        1
      ],
      "name": "Gustavo Delhon"
    },
    {
      "affs": [
        2
      ],
      "name": "María Cruz Miraglia"
    },
    {
      "affs": [
        3
      ],
      "name": "Sabrina Galdo-Novo"
    },
    {
      "affs": [
        3
      ],
      "name": "Ana Taffarel"
    },
    {
      "affs": [
        0
      ],
      "name": "Byung-Joon Seung"
    },
    {
      "affs": [
        0
      ],
      "name": "Sushil Khatiwada"
    },
    {
      "affs": [
        2
      ],
      "name": "Daniel Mariano Pérez-Filgueira"
    },
    {
      "affs": [
        0
      ],
      "name": "Daniel L. Rock",
      "orcid": "0000-0002-8548-3317"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3yg2ek9r8b69s.bib",
  "cited_by": 0,
  "doi": "10.1099/jgv.0.002344",
  "fulltext_url": "https://europepmc.org/articles/PMC13727147",
  "id": "3yg2ek9r8b69s",
  "issns": [
    "0022-1317",
    "1465-2099"
  ],
  "landing_url": "https://europepmc.org/article/MED/42970347",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Short Communication"
    ],
    [
      "openalex",
      "1102|Animal Disease Management and Epidemiology"
    ]
  ],
  "openalex": "W7163572485",
  "pdf_url": "https://europepmc.org/articles/PMC13727147?pdf=render",
  "pmcid": "PMC13727147",
  "pmid": "42970347",
  "published": "2026-11-01",
  "publisher": "Microbiology Society",
  "ris": "https://research.draconitic.com/paper/3yg2ek9r8b69s.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:44Z",
      "native_id": "MED:42970347",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42970347"
    },
    {
      "first_seen": "2026-11-05T12:23:17Z",
      "native_id": "PMC13727147",
      "source": "pmc",
      "url": "https://doi.org/10.1099/jgv.0.002344"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Animal Disease Management and Epidemiology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Novel recombinant bovine papular stomatitis virus expressing foot-and-mouth disease virus-like particles elicits neutralizing antibodies in cattle.",
  "title_text": "Novel recombinant bovine papular stomatitis virus expressing foot-and-mouth disease virus-like particles elicits neutralizing antibodies in cattle.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:22:53Z",
  "url": "https://research.draconitic.com/paper/3yg2ek9r8b69s",
  "venue": "The Journal of general virology",
  "versions": []
}