{
  "abstract": "Rapid and accurate diagnosis of bacterial infections is essential for effective clinical management, food safety, and environmental monitoring. Traditional culture-based methods are time-consuming and inadequate for addressing urgent challenges such as antimicrobial resistance (AMR). This review categorizes recent bacterial detection strategies into three major biomarker types: genetic, protein, and enzymatic. For each category, we outline the underlying detection mechanisms, evaluate analytical performance, and highlight representative technologies, including polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) assays, advanced immunoassays, aptamer sensors, and enzyme-based assays. Meanwhile, we provide a detailed description of representative examples for each representative technology. Key metrics such as sensitivity, detection time, portability, and point-of-care (POC) applicability are compared to reveal the strengths and limitations of each approach. Translational progress is discussed with emphasis on commercialized platforms, field-deployable devices, and multimodal workflows. Finally, major technical bottlenecks are identified, and future directions are proposed for developing robust, rapid, and widely accessible bacterial diagnostics.",
  "abstract_shown": true,
  "affiliations": [
    "Guangdong Provincial Key Laboratory of New Drug Screening & NMPA Key Laboratory for Research and Evaluation of Drug Metabolism & Guangdong-Hongkong-Macao Joint Laboratory for New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China",
    "Corresponding author."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Hanren Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiayi Xiao"
    },
    {
      "affs": [
        0
      ],
      "name": "Bihong Diao"
    },
    {
      "affs": [
        0
      ],
      "name": "Shiquan Zheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Huaze Shao"
    },
    {
      "affs": [
        1
      ],
      "name": "Lihong Liu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/40bkynfgvmmxq.bib",
  "cited_by": 5,
  "doi": "10.1016/j.jpha.2026.101584",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2095177926000390",
  "id": "40bkynfgvmmxq",
  "issns": [
    "2095-1779",
    "2214-0883"
  ],
  "landing_url": "https://doi.org/10.1016/j.jpha.2026.101584",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RM1-950"
    ],
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ]
  ],
  "openalex": "W7128440305",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-24",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/40bkynfgvmmxq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:44:30Z",
      "native_id": "5237ec220b924536b38da03c52b62a4d",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.jpha.2026.101584"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:RM1-950",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Recent advances in bacterial detection techniques targeting genetic, protein, and enzymatic biomarkers",
  "title_text": "Recent advances in bacterial detection techniques targeting genetic, protein, and enzymatic biomarkers",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:44:30Z",
  "url": "https://research.draconitic.com/paper/40bkynfgvmmxq",
  "venue": "Journal of Pharmaceutical Analysis",
  "versions": []
}