{
  "abstract": "This study aimed to qualitatively and quantitatively characterize the gene and protein expression of cannabinoid receptors type 1 (CB1) and 2 (CB2), and to describe their cellular distribution in the urinary bladder of cats. Samples were collected post-mortem from 11 cats with no history of lower urinary tract disease and were analyzed using reverse-transcription quantitative PCR (RT-qPCR), Western blot, and immunofluorescence. Mean fluorescence intensity was analyzed in the urothelium and detrusor muscle using a paired t-test (p < 0.05). CNR2 transcripts were detected in both compartments, whereas CNR1 transcripts were not detectable. Western blot detected both CB1 and CB2 bands. CB1R immunoreactivity (CB1R-IR) was sparse within the urothelium, with occasional umbrella and basal cells, and was detected in endothelial cells, intramural neurons, nerve fibers, and detrusor smooth muscle cells. CB1R-IR was significantly higher in the detrusor muscle (p = 0.0006). CB2R-IR showed marked staining in umbrella cells and faint staining in basal cells; it was also observed in bundles of nerve fibers, intramural neurons, detrusor smooth muscle cells, and vascular structures. CB2R-IR was significantly higher in the urothelium (p < 0.0001). These findings provide a molecular basis for studies investigating the endocannabinoid system as a potential therapeutic target in feline lower urinary tract disorders.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy",
    "Department of Veterinary Medicine, University of Teramo, 64100 Teramo, Italy",
    "Independent Researcher, São Paulo 01307-000, Brazil",
    "RI.MOS Medical Products, 41037 Mirandola, Italy"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Rodrigo Zamith Cunha",
      "orcid": "0000-0002-8548-6905"
    },
    {
      "affs": [
        0
      ],
      "name": "Augusta Zannoni",
      "orcid": "0000-0002-2972-4921"
    },
    {
      "affs": [
        0
      ],
      "name": "Giulia Biondolillo"
    },
    {
      "affs": [
        0
      ],
      "name": "Chiara Bernardini",
      "orcid": "0000-0003-3384-0244"
    },
    {
      "affs": [
        2
      ],
      "name": "Lia Nasi"
    },
    {
      "affs": [
        3
      ],
      "name": "Chiara Di Placido"
    },
    {
      "affs": [
        0
      ],
      "name": "Roberto Chiocchetti",
      "orcid": "0000-0003-4448-6199"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/40q6q40de2tc9.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219910",
  "fulltext_url": null,
  "id": "40q6q40de2tc9",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9910",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/40q6q40de2tc9.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T00:21:14Z",
      "native_id": "10.3390/ijms27219910",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9910"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Cellular Distribution and Expression of Cannabinoid Receptors Type 1 and 2 in the Urinary Bladder of Cats (Felis catus)",
  "title_text": "Cellular Distribution and Expression of Cannabinoid Receptors Type 1 and 2 in the Urinary Bladder of Cats (Felis catus)",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T00:21:12Z",
  "url": "https://research.draconitic.com/paper/40q6q40de2tc9",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}