{
  "abstract": "High-speed three-dimensional imaging in scattering biological tissues remains challenging because volumetric microscopy generally requires scanning, whereas snapshot light-field approaches divide limited detector pixels among multiple views. This constraint is particularly severe in the second near-infrared window (NIR-II), where InGaAs cameras have small sensor formats and high detector noise. Here we introduce NIR-II squeezed light-field microscopy (NIR-II SLIM), which optically rotates and compresses multiple perspective views before detection, allowing efficient use of camera pixels while retaining complementary spatial information for three-dimensional reconstruction. NIR-II SLIM acquires up to 600 volumes s-1 with a reconstructed lateral sampling grid of 512 x 512 pixels. We use this method for label-free imaging of cardiac dynamics in pigmented late-larval zebrafish, resolving chamber deformation and millisecond-scale atrioventricular-valve motion, and for NIR-II fluorescence imaging of vascular and lymphatic transport in mice. NIR-II SLIM provides a detector-efficient approach for high-speed volumetric imaging of rapid biological dynamics in scattering tissues.",
  "abstract_shown": true,
  "affiliations": [
    "University of California, Los Angeles"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "D. Y. Kim"
    },
    {
      "name": "Z. Zang"
    },
    {
      "name": "E. Y. Lin"
    },
    {
      "name": "R. Zhao"
    },
    {
      "name": "J. Wang"
    },
    {
      "name": "C. Y. Kam"
    },
    {
      "name": "T. Hsiai"
    },
    {
      "name": "E. M. Sletten"
    },
    {
      "affs": [
        0
      ],
      "name": "L. Gao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/41jt4mq1nyhhd.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.13.744709",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.08.13.744709v2.full",
  "id": "41jt4mq1nyhhd",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.08.13.744709v2",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "openalex",
      "2741|Optical Imaging and Spectroscopy Techniques"
    ]
  ],
  "openalex": "W7203530916",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.08.13.744709v2.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/41jt4mq1nyhhd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:08Z",
      "native_id": "10.64898/2026.08.13.744709",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.08.13.744709v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "NIR-II squeezed light-field microscopy enables high-speed volumetric imaging of deep-tissue dynamics in vivo",
  "title_text": "NIR-II squeezed light-field microscopy enables high-speed volumetric imaging of deep-tissue dynamics in vivo",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:08Z",
  "url": "https://research.draconitic.com/paper/41jt4mq1nyhhd",
  "venue": "bioRxiv",
  "versions": []
}