{
  "abstract": "Mammalian orthoreovirus (MRV), a member of the family Spinareoviridae within the order Reovirales, has a ten-segmented double-stranded RNA genome and is known for its environmental stability and broad host range, including humans. Although human MRV infections are usually asymptomatic or mild, occasional severe MRV-associated disease has been reported. Despite the widespread presence of MRV, particularly in contaminated water, genomic data from South America are limited, especially from environmental sources in underserved urban settlements. In this study, we isolated a virus from a heavily polluted stream running through an underprivileged, underserved, urban settlement in São Paulo, Brazil. The causative agent was identified as a novel MRV strain (MRV1_SP/BR) by whole-genome Illumina sequencing, and its presence was subsequently confirmed by RT-qPCR. This analysis revealed a complex mosaic genome architecture indicative of genetic reassortment. Based on phylogenetic analysis of the S1 segment, the strain was genetically classified as MRV-1, whereas its remaining segments showed high nucleotide identity to sequences previously detected in humans and bats. These findings provide the first genomic characterization of a reassortant MRV detected in an urban stream in South America and expand the available information on MRV diversity in sewage-impacted aquatic environments.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Medical Investigation in Virology (LIM-52), Instituto de Medicina Tropical de São Paulo, Faculdade de Medicina, Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, São Paulo 05403-000, Brazil.",
    "Department of Infectology and Tropical Medicine, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, Brazil.",
    "Department of Environmental Health, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil.",
    "NARA-Center for Research in Environmental Risk Assessment, São Paulo 01246-904, Brazil.",
    "Department of Environmental Analysis, Division of Microbiology and Parasitology, Environmental Company of the State of São Paulo (CETESB), São Paulo 05459-900, Brazil."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Anderson Vicente de Paula"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Layla Honorato",
      "orcid": "0000-0001-7084-3095"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Lucy Santos Vilas Boas"
    },
    {
      "affs": [
        1
      ],
      "name": "Anna Sara Shafferman Levin"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Maria Tereza Pepe Razzolini",
      "orcid": "0000-0003-3308-9550"
    },
    {
      "affs": [
        4
      ],
      "name": "Ronalda Silva de Araújo",
      "orcid": "0000-0003-4248-4990"
    },
    {
      "affs": [
        4
      ],
      "name": "Mikaela Renata Funada Barbosa",
      "orcid": "0000-0002-7730-9247"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Antonio Charlys da Costa",
      "orcid": "0000-0001-5516-5177"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Maria Cassia Mendes-Correa"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/41mnkk8gh1tfn.bib",
  "cited_by": 0,
  "doi": "10.3390/v18101161",
  "fulltext_url": "https://europepmc.org/articles/PMC13709415",
  "id": "41mnkk8gh1tfn",
  "issns": [
    "1999-4915"
  ],
  "landing_url": "https://europepmc.org/article/MED/42944080",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2725|Viral gastroenteritis research and epidemiology"
    ]
  ],
  "openalex": "W7222929220",
  "pdf_url": "https://europepmc.org/articles/PMC13709415?pdf=render",
  "pmcid": "PMC13709415",
  "pmid": "42944080",
  "published": "2026-10-19",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/41mnkk8gh1tfn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42944080",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42944080"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Viral gastroenteritis research and epidemiology",
      "subfield": "infectious-disease"
    }
  ],
  "title": "First Identification and Genomic Characterization of Mammalian Orthoreovirus Type 1 in a Heavily Polluted Stream from an Underserved Urban Settlement in Brazil.",
  "title_text": "First Identification and Genomic Characterization of Mammalian Orthoreovirus Type 1 in a Heavily Polluted Stream from an Underserved Urban Settlement in Brazil.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/41mnkk8gh1tfn",
  "venue": "Viruses",
  "versions": []
}