{
  "abstract": "Background\n\nSustained tachyarrhythmia is a major cause of cardiac dysfunction and can lead to tachycardia-induced cardiomyopathy (TIC). However, the role of small extracellular vesicle (sEV)-mediated intercellular communication in the pathogenesis of TIC remains poorly understood. We aimed to investigate whether sEVs derived from tachy-paced cardiomyocytes affect intracellular Ca²⁺ handling in recipient cardiomyocytes.Methods and results\n\nCultured murine atrial cardiomyocytes (HL-1 cells) were subjected to high-frequency electrical stimulation (tachy-pacing, 10 Hz), and sEVs were isolated from the culture supernatant. sEVs derived from the tachy-paced cells and non-paced control cells were subsequently applied to neonatal mouse cardiomyocytes (NMCMs). Intracellular Ca²⁺ transients, sarcoplasmic reticulum (SR) Ca²⁺ content, and the expression of Ca²⁺-handling proteins were evaluated. High-frequency electrical stimulation significantly increased sEV secretion from HL-1 cells. sEVs derived from tachy-paced HL-1 cells markedly slowed Ca²⁺ decay and reduced SERCA2a protein expression in recipient NMCMs.Conclusions\n\nOur study demonstrates that sEVs derived from tachy-paced cardiomyocytes suppress SERCA2a expression, thereby leading to impaired SR Ca²⁺ reuptake and prolonged Ca²⁺ transients. These findings suggest that sEV-mediated signaling may contribute to Ca²⁺ dysregulation during sustained tachycardia.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cardiovascular Medicine, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo, Japan.",
    "Department of Clinical and Diagnostic Laboratory Science, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo, Japan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lai Wei"
    },
    {
      "affs": [
        0
      ],
      "name": "Satoshi Iwamiya"
    },
    {
      "affs": [
        0
      ],
      "name": "Kohei Kawajiri"
    },
    {
      "affs": [
        0
      ],
      "name": "Junji Yamaguchi"
    },
    {
      "affs": [
        1
      ],
      "name": "Minori Kurata"
    },
    {
      "affs": [
        0
      ],
      "name": "Kensuke Ihara"
    },
    {
      "affs": [
        0
      ],
      "name": "Tetsuo Sasano"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/42xwjwpyh8r0c.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jmccpl.2026.100874",
  "fulltext_url": "https://europepmc.org/articles/PMC13695238",
  "id": "42xwjwpyh8r0c",
  "issns": [
    "2772-9761"
  ],
  "landing_url": "https://europepmc.org/article/MED/42927336",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7221047026",
  "pdf_url": "https://europepmc.org/articles/PMC13695238?pdf=render",
  "pmcid": "PMC13695238",
  "pmid": "42927336",
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/42xwjwpyh8r0c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:18Z",
      "native_id": "MED:42927336",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42927336"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Tachy-paced cardiomyocyte-derived small extracellular vesicles impair SERCA2a-dependent Ca²⁺ handling in recipient cardiomyocytes.",
  "title_text": "Tachy-paced cardiomyocyte-derived small extracellular vesicles impair SERCA2a-dependent Ca²⁺ handling in recipient cardiomyocytes.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:18Z",
  "url": "https://research.draconitic.com/paper/42xwjwpyh8r0c",
  "venue": "Journal of molecular and cellular cardiology plus",
  "versions": []
}