{
  "abstract": "Heparin is a sulfated glycosaminoglycan, and the most used pharmaceutical anticoagulant worldwide. Heparin is isolated primarily from porcine sources, and possesses a complex variable structure, wherein anticoagulant activity is principally derived from the pentasaccharide GlcNS6S-GlcA-GlcNS3S6S-IdoA2S-GlcNS6S (AGA*IA) motif, containing an infrequent 3-O-sulfated glucosamine that enhances antithrombin stabilization. Pedobacter heparinus heparin lyases are currently widely employed for characterization of heparin and its derivatives, but have limited activity near regions of 3-O-sulfation, hindering analyses of anticoagulation relevant sequences. Here, using a panel of biophysical and structural techniques, we show that Bacteroides eggerthii lyase I liberates ΔUA-GlcNS3S6S disaccharides from the defined pentasaccharide, fondaparinux. This activity provides improved access to 3-O-sulfated heparin standards, with potential to aid heparin quality control.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "Taran K Kandola"
    },
    {
      "name": "Courtney J Mycroft-West"
    },
    {
      "name": "Marcelo A Lima"
    },
    {
      "name": "Abigail Turner"
    },
    {
      "name": "Cristina Gardini"
    },
    {
      "name": "Elena Urso"
    },
    {
      "name": "Thomas A Torode"
    },
    {
      "name": "Gavin J Miller"
    },
    {
      "name": "Antonella Bisio"
    },
    {
      "name": "Edwin A Yates"
    },
    {
      "name": "Marco Guerrini"
    },
    {
      "name": "Mark A Skidmore"
    },
    {
      "name": "Liang Wu",
      "orcid": "0000-0003-0294-7065"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/42zc85ekcdrzp.bib",
  "cited_by": 0,
  "doi": "10.1093/glycob/cwag097",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13714783/",
  "id": "42zc85ekcdrzp",
  "issns": [
    "0959-6658",
    "1460-2423"
  ],
  "landing_url": "https://doi.org/10.1093/glycob/cwag097",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "AcademicSubjects/SCI01000"
    ],
    [
      "heading",
      "Communication"
    ],
    [
      "heading",
      "SfG 2025 Meeting"
    ],
    [
      "openalex",
      "1307|Proteoglycans and glycosaminoglycans research"
    ]
  ],
  "openalex": "W7221702238",
  "pdf_url": "https://academic.oup.com/glycob/advance-article-pdf/doi/10.1093/glycob/cwag097/71908143/cwag097.pdf",
  "pmcid": "PMC13714783",
  "pmid": "42867289",
  "published": "2026-10-13",
  "publisher": "Oxford University Press",
  "ris": "https://research.draconitic.com/paper/42zc85ekcdrzp.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:22:13Z",
      "native_id": "PMC13714783",
      "source": "pmc",
      "url": "https://doi.org/10.1093/glycob/cwag097"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Proteoglycans and glycosaminoglycans research",
      "subfield": "cell-biology"
    }
  ],
  "title": "Functional characterization of a 3-O-sulfate tolerant heparin lyase from Bacteroides eggerthii",
  "title_text": "Functional characterization of a 3-O-sulfate tolerant heparin lyase from Bacteroides eggerthii",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:13Z",
  "url": "https://research.draconitic.com/paper/42zc85ekcdrzp",
  "venue": "Glycobiology",
  "versions": []
}