{
  "abstract": "Lithium carbonate (Li2CO3) is a first-line pharmacotherapy for bipolar disorder (BPD) in female patients of childbearing age, although it is associated with embryonic teratogenicity. Lithium exposure in batteries and pharmaceuticals has been a toxicity factor for women’s reproductive health in energy, environment, and fitness fields. Neural tube defects (NTDs) are the severest reproductive outcome of maternal Li2CO3 exposure. Currently, there is a lack of effective prevention for Li2CO3 neurotoxicity. Our previous study revealed that lithium-induced murine NTDs correlated with enhanced proliferation and impaired inositol biosynthesis. In this study, 400 mg/kg inositol was used to treat the lithium-induced murine NTDs after calculating the toxicity using ADMETlab 3.0 and Deep-PK. We found that inositol supplementation ameliorated fetal neural phenotypes, crown-rump length, and body weight and rescued aberrant cell proliferation and dysregulated the GSK3β/β-catenin/CyclinD1 pathway in both animal and cellular models. The affinity of GSK3β-inositol interaction was analyzed and validated using molecular dynamics and surface plasmon resonance. GSK3β-edited cell models further revealed that inositol altered GSK3β post-transcriptional status and phosphorylation, consequently modulating the GSK3β/β-catenin/CyclinD1 signaling cascade. Overall, our study clarified that inositol alleviates lithium-induced NTDs via the GSK3β/β-catenin/CyclinD1 pathway, revealed the direct molecular interaction between inositol and GSK3β, and highlighted the potential of inositol as a therapeutic prevention against lithium-induced embryonic neurotoxicity for childbearing female BPD patients.",
  "abstract_shown": true,
  "affiliations": [
    "Capital Center for Children’s Health, Capital Medical University, Capital Institute of Pediatrics, No. 2 Yabao Road, Chaoyang District, Beijing 100020, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shen Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Jin Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiuwei Wang",
      "orcid": "0000-0001-5143-0826"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhen Guan"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhiqiang Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Aiyun Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jianhua Wang",
      "orcid": "0000-0002-0586-4372"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/43dm4q42zdmjg.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219785",
  "fulltext_url": null,
  "id": "43dm4q42zdmjg",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9785",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/43dm4q42zdmjg.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:54Z",
      "native_id": "10.3390/ijms27219785",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9785"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Inositol-Dependent Alleviation of Lithium-Induced Embryonic Neurotoxicity in Mice Through the GSK3β/β-Catenin/CyclinD1 Pathway",
  "title_text": "Inositol-Dependent Alleviation of Lithium-Induced Embryonic Neurotoxicity in Mice Through the GSK3β/β-Catenin/CyclinD1 Pathway",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T00:20:52Z",
  "url": "https://research.draconitic.com/paper/43dm4q42zdmjg",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}