{
  "abstract": "Poly(3-hydroxybutyrate) (PHB) is a biodegradable polyester considered a sustainable alternative to conventional plastics. This study aimed to isolate a PHB-producing bacterium from agricultural soil and to evaluate the natural honey produced by Apis mellifera as an alternative carbon source for PHB production. A PHB-producing isolate was obtained and identified as Bacillus subtilis strain MUST-MWS1 based on morphological, biochemical, and 16 S rRNA gene analyses. Cultures were grown at pH 8.2 using LB medium, glucose, or honey as the main carbon source, and PHB was extracted by a chloroform–methanol method. The polymer was characterized by Fourier transform infrared spectroscopy, gas chromatography–mass spectrometry, X-ray diffraction, differential scanning calorimetry, and thermogravimetric analysis. Honey yielded the highest PHB titer (0.2669 ± 0.0078 g/L), compared with glucose (0.2168 ± 0.0068 g/L) and LB medium (0.1933 ± 0.0067 g/L). The structural and thermal properties of the biosynthesized PHB were consistent with those reported for commercial PHB. These findings indicate that B. subtilis MUST-MWS1 is a promising PHB producer and that honey can serve as an effective alternative carbon source for bioplastic production.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Medical Biotechnology, College of Biotechnology, Misr University for Science and Technology (MUST), Giza, 12566 Egypt",
    "Industrial Pharmacy Department, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology (MUST), 12566 Giza, Egypt",
    "Department of Environmental Biotechnology, College of Biotechnology, Misr University for Science and Technology (MUST), 12566 Giza, Egypt",
    "Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, 12613 Egypt",
    "Department of Pharmaceutical Biotechnology, College of Biotechnology, Misr University for Science and Technology (MUST), 12566 Giza, Egypt"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Afaf Altrawy",
      "orcid": "0000-0001-9736-6401"
    },
    {
      "affs": [
        1
      ],
      "name": "Rehab Abdelmonem"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Mahmoud W. Sadik"
    },
    {
      "affs": [
        2
      ],
      "name": "Sara K. Mostafa"
    },
    {
      "affs": [
        2
      ],
      "name": "Shrouk R. Elsaid"
    },
    {
      "affs": [
        4
      ],
      "name": "Mohamed A. Abd-Elhakeem"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/43j9tqzf0nn4h.bib",
  "cited_by": 0,
  "doi": "10.1186/s12866-026-05715-z",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13732992/",
  "id": "43j9tqzf0nn4h",
  "issns": [
    "1471-2180"
  ],
  "landing_url": "https://doi.org/10.1186/s12866-026-05715-z",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "2502|biodegradable polymer synthesis and properties"
    ]
  ],
  "openalex": "W7225174234",
  "pdf_url": "https://link.springer.com/content/pdf/10.1186/s12866-026-05715-z.pdf",
  "pmcid": "PMC13732992",
  "pmid": "42977914",
  "published": "2026-11-03",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/43j9tqzf0nn4h.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:22:52Z",
      "native_id": "PMC13732992",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s12866-026-05715-z"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2502|biodegradable polymer synthesis and properties",
      "subfield": "materials-polymers"
    }
  ],
  "title": "A newly Bacillus subtilis isolate for PHB biosynthesis: optimization and thermal spectroscopic validation",
  "title_text": "A newly Bacillus subtilis isolate for PHB biosynthesis: optimization and thermal spectroscopic validation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:22:52Z",
  "url": "https://research.draconitic.com/paper/43j9tqzf0nn4h",
  "venue": "BMC Microbiology",
  "versions": []
}