{
  "abstract": "This study aims to investigate the role of cuproptosis, a novel form of regulated cell death, in the transition from acute kidney injury (AKI) to chronic kidney disease (CKD). Multi-omics approaches, including single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, and in vitro experiments based on cells, were integrated to dissect the spatiotemporal regulation of cuproptosis in AKI. Podocytes exhibited the highest cuproptosis activity. A critical intercellular communication axis was identified, wherein endothelial cell-derived GAS6 signals through the podocyte receptors AXL/TYRO3. The transcription factor CEBPD was upregulated early in AKI and was found to positively regulate the GAS6-related pathway, with its target genes enriched in TGF-β and JAK-STAT signaling, ultimately leading to receptor activation and cuproptosis. Spatially, CEBPD activation preceded the increase in AXL, followed by late-phase upregulation of GAS6. The alteration in AXL protein expression was confirmed in vitro.Our study reveals a CEBPD-mediated transcriptional program that drives podocyte cuproptosis by transactivating the AXL receptor, providing novel mechanistic insights into the AKI-to-CKD transition.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Nephrology, The First People’s Hospital of Nanning, Nanning, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jingmei Liang"
    },
    {
      "name": "Yuyun Hu"
    },
    {
      "name": "Bing Liao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/44bhpsxss09nx.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0359878",
  "fulltext_url": null,
  "id": "44bhpsxss09nx",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0359878",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2727|Acute Kidney Injury Research"
    ],
    [
      "plos",
      "/Biology and life sciences/Anatomy/Renal system/Kidneys"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/DNA-binding proteins/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Regulatory proteins/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Computational biology/Genome analysis/Transcriptome analysis"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Transcriptional control"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Genomics/Genome analysis/Transcriptome analysis"
    ],
    [
      "plos",
      "/Computer and information sciences/Network analysis/Signaling networks"
    ],
    [
      "plos",
      "/Medicine and health sciences/Anatomy/Renal system/Kidneys"
    ],
    [
      "plos",
      "/Medicine and health sciences/Nephrology/Renal diseases/Chronic kidney disease"
    ]
  ],
  "openalex": "W7221196990",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0359878&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-09",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/44bhpsxss09nx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:30Z",
      "native_id": "10.1371/journal.pone.0359878",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0359878"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
    },
    {
      "field": "medicine",
      "origin": "plos:anatomy",
      "subfield": "physiology"
    }
  ],
  "title": "Intercellular GAS6-AXL signaling triggers podocyte cuproptosis in acute kidney injury upstream regulated by CEBPD",
  "title_text": "Intercellular GAS6-AXL signaling triggers podocyte cuproptosis in acute kidney injury upstream regulated by CEBPD",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:30Z",
  "url": "https://research.draconitic.com/paper/44bhpsxss09nx",
  "venue": "PLOS One",
  "versions": []
}