{
  "abstract": "BMP9 is an important regulator in angiogenesis, tissue development, and immune response. Here, we present a protocol to examine the regulatory mechanism of unprocessed BMP9 precursor in BMP9 signaling. We describe procedures for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo. We describe steps for their assessment using flow cytometric analysis, C2C12 differentiation assay, chicken embryo chorioallantoic membrane assay, and mouse subcutaneous Matrigel assay. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, P.R. China.",
    "School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, P.R. China; State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, P.R. China. Electronic address: qinxiaohong@tju.edu.cn.",
    "School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, P.R. China; State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, P.R. China. Electronic address: lizhi.mi@tju.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jianing Duan"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuanyuan Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Weida Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaohong Qin"
    },
    {
      "affs": [
        2
      ],
      "name": "Li-Zhi Mi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/44n1nhwv5ysbp.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104894",
  "fulltext_url": "https://europepmc.org/articles/PMC13686000",
  "id": "44n1nhwv5ysbp",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42868000",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|TGF-β signaling in diseases"
    ]
  ],
  "openalex": "W7221668364",
  "pdf_url": "https://europepmc.org/articles/PMC13686000?pdf=render",
  "pmcid": "PMC13686000",
  "pmid": "42868000",
  "published": "2026-10-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/44n1nhwv5ysbp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42868000",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42868000"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|TGF-β signaling in diseases",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo.",
  "title_text": "Protocol for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/44n1nhwv5ysbp",
  "venue": "STAR protocols",
  "versions": []
}