{
  "abstract": "Light-cycle time-restricted feeding disrupts daily cardiovascular and thermoregulatory rhythms, but the molecular effects of light-cycle time-restricted feeding on the heart have been measured only at the level of total gene expression. We used Oxford Nanopore long-read RNA sequencing to resolve the full-length ventricular transcriptome from male and female mice under ad libitum feeding or light-cycle time-restricted feeding across the 24-hour cycle. Greater than 20% of cardiac transcripts represent unannotated variants of known genes absent from the current GENCODE reference annotation. Light-cycle time-restricted feeding reorganizes transcript usage across hundreds of genes, including genes encoding splicing regulators, largely without changing total gene expression. The genes affected are sex-specific, with fewer than 2% of changes shared at the gene, transcript, and transcript-usage levels. We show that transcript-level regulation is a previously underrecognized component of the cardiac response to altered feeding behavior, undetected by conventional short-read approaches.",
  "abstract_shown": true,
  "affiliations": [
    "University of Kentucky"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "S. Naidu"
    },
    {
      "name": "A. Prabhat"
    },
    {
      "name": "B. Peck"
    },
    {
      "name": "T. S. Seward"
    },
    {
      "name": "E. Schroder"
    },
    {
      "name": "B. Delisle"
    },
    {
      "affs": [
        0
      ],
      "name": "Y. Wen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/452z7e9w5xk7d.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.22.753546",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753546v1.full",
  "id": "452z7e9w5xk7d",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753546v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "physiology"
    ],
    [
      "openalex",
      "2737|Dietary Effects on Health"
    ]
  ],
  "openalex": "W7214637499",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753546v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/452z7e9w5xk7d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:58Z",
      "native_id": "10.64898/2026.09.22.753546",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753546v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:physiology",
      "subfield": "physiology"
    }
  ],
  "title": "Light-cycle time-restricted feeding remodels a hidden layer of the cardiac transcriptome through sex-specific transcript switching",
  "title_text": "Light-cycle time-restricted feeding remodels a hidden layer of the cardiac transcriptome through sex-specific transcript switching",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:58Z",
  "url": "https://research.draconitic.com/paper/452z7e9w5xk7d",
  "venue": "bioRxiv",
  "versions": []
}