{
  "abstract": "G-patch proteins are emerging as key regulatory cofactors of DEAH-box RNA helicases involved in pre-mRNA splicing, yet the functions of many family members remain poorly understood. Here, we characterize the conserved G-patch domain–containing protein Cwf28 from Schizosaccharomyces pombe, an essential factor with a previously unclear molecular function, and define its role within the spliceosome. Tandem affinity purification coupled with mass spectrometry revealed that Cwf28 predominantly associates with components of the Prp19 complex (NTC) and factors involved in the catalytic activation and progression of the spliceosome, placing it within catalytically active spliceosomal assemblies. Gene ontology analysis showed enrichment of the Cwf28 interactome in factors involved in spliceosome assembly, activation, and catalytic remodeling. Notably, the DEAH-box RNA helicase Cdc28, the ortholog of human DHX16 and Saccharomyces cerevisiae Prp2, was identified as the most abundant interactor. Further analysis demonstrated that Cwf28 interacts with Cdc28 via its conserved G-patch domain, and domain mapping confirmed that this interaction is G-patch domain dependent. Together, these findings identify Cwf28 as a component of catalytically active spliceosomes and suggest a potential role for Cwf28 in modulating the activity of the RNA helicase Cdc28, providing insight into conserved mechanisms underlying RNA helicase regulation during splicing.",
  "abstract_shown": true,
  "affiliations": [
    "Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia",
    "Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden",
    "Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Laura Olivia Karika"
    },
    {
      "affs": [
        1
      ],
      "name": "Ingrid Cipakova"
    },
    {
      "affs": [
        2
      ],
      "name": "Miroslava Kretova"
    },
    {
      "name": "Tomas Selicky"
    },
    {
      "name": "Natalia Cmikova"
    },
    {
      "name": "Daniela Nemcekova"
    },
    {
      "name": "Lenka Kohutova"
    },
    {
      "name": "Peter Barath"
    },
    {
      "name": "Lubos Cipak"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/454cyxy805z89.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0357276",
  "fulltext_url": null,
  "id": "454cyxy805z89",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0357276",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Enzymology/Enzymes/Helicases"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Enzymology/Enzymes/Helicases/RNA helicases"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/RNA processing/RNA splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/Spliceosomes"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Enzymes/Helicases"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Enzymes/Helicases/RNA helicases"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Post-translational modification/Phosphorylation"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Protein interactions"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/RNA processing/RNA splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Fungi/Yeast/Saccharomyces/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Fungi/Yeast/Schizosaccharomyces/Schizosaccharomyces pombe"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Schizosaccharomyces pombe"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Yeast and fungal models/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Yeast and fungal models/Schizosaccharomyces pombe"
    ],
    [
      "plos",
      "/Research and analysis methods/Model organisms/Saccharomyces cerevisiae"
    ],
    [
      "plos",
      "/Research and analysis methods/Model organisms/Schizosaccharomyces pombe"
    ]
  ],
  "openalex": "W7207611920",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0357276&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-03",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/454cyxy805z89.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "10.1371/journal.pone.0357276",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0357276"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:enzymology",
      "subfield": "enzymology-metabolism"
    },
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
    }
  ],
  "title": "The G-patch protein Cwf28 interacts with the RNA helicase Cdc28 in catalytically active spliceosomes in Schizosaccharomyces pombe",
  "title_text": "The G-patch protein Cwf28 interacts with the RNA helicase Cdc28 in catalytically active spliceosomes in Schizosaccharomyces pombe",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/454cyxy805z89",
  "venue": "PLOS One",
  "versions": []
}