{
  "abstract": "Most RNA-cleaving DNAzymes are limited to cleaving between combinations of the four canonical ribonucleotides. Through in vitro selection, we isolated ICD11-1T, a DNAzyme that selectively cleaves at inosine, a non-canonical RNA modification generated by adenosine deamination. ICD11-1T cleaves inosine-containing substrates with a rate constant of 0.1 min⁻¹ and achieves >10³-fold selectivity over adenosine, discriminating between nucleosides that differ at the C6 and N1 positions of the purine ring. Molecular dynamics simulations revealed that the inosine positions the cleavage site near the catalytic core, whereas the adenosine complex sequesters it away, accounting for the stringent specificity. Using ICD11-1T, we constructed a fluorescent biosensor that enabled imaging of endogenous ADAR (adenosine deaminase acting on RNA) editing activity in fixed and permeabilized cells. This work provides a proof-of-concept tool for studying adenosine-to-inosine (A-to-I) RNA editing and establishes a strategy for expanding the functionality of DNAzymes.",
  "abstract_shown": true,
  "affiliations": [
    "Central Hospital of Dalian University of Technology, Dalian Medical-Engineering Interdisciplinary Rapid Diagnosis of Major Diseases Laboratory, Dalian University of Technology Dalian 116000 China yuanhonglab@163.com lijiuxing@dlut.edu.cn mliu@dlut.edu.cn.",
    "School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology Dalian 116024 China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Shusen Xiao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Pan Jia"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Kai Niu"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaoqian Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaozheng Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yangyang Chang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zijie Zhang",
      "orcid": "0000-0003-2757-3071"
    },
    {
      "affs": [
        0
      ],
      "name": "Hong Yuan"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiuxing Li",
      "orcid": "0000-0001-7012-2482"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Meng Liu",
      "orcid": "0000-0003-3114-9003"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/456xjjkxg4rh5.bib",
  "cited_by": 0,
  "doi": "10.1039/d6sc06852a",
  "fulltext_url": "https://europepmc.org/articles/PMC13682592",
  "id": "456xjjkxg4rh5",
  "issns": [
    "2041-6520",
    "2041-6539"
  ],
  "landing_url": "https://europepmc.org/article/MED/42904253",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|DNA and Nucleic Acid Chemistry"
    ]
  ],
  "openalex": "W7222936548",
  "pdf_url": "https://europepmc.org/articles/PMC13682592?pdf=render",
  "pmcid": "PMC13682592",
  "pmid": "42904253",
  "published": "2026-10-19",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/456xjjkxg4rh5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42904253",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42904253"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|DNA and Nucleic Acid Chemistry",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Exploiting inosine as a distinctive cleavage site for deriving a highly specific RNA-cleaving DNAzyme.",
  "title_text": "Exploiting inosine as a distinctive cleavage site for deriving a highly specific RNA-cleaving DNAzyme.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/456xjjkxg4rh5",
  "venue": "Chemical science",
  "versions": []
}