{
  "abstract": "Fruit skin color is a decisive agronomic trait governing the commercial value of peaches; although PpMYB10.1 is known to regulate anthocyanin accumulation in peach, the specific promoter structural variant that silences PpMYB10.1 expression in non-red skin types has not been identified and functionally validated. Here, using a first backcross (BC1) population, we integrated bulked segregant analysis sequencing (BSA-seq), transcriptome profiling, and whole-genome resequencing to map the causal locus to a 453.083-kb interval on chromosome 3 harboring the R2R3-MYB transcription factor PpMYB10.1. Transient overexpression and virus-induced gene silencing (VIGS), together with stable tobacco transformation, confirmed that PpMYB10.1 regulates the non-red skin phenotype in this population. Sequence comparison revealed a 26-bp insertion in the PpMYB10.1 promoter of non-red fruit; reporter assays demonstrated that this insertion strongly reduces promoter activity and silences PpMYB10.1 expression. A co-dominant InDel marker co-segregated with the skin color phenotype in all tested accessions and populations. Our findings suggest a promoter structural variant is associated with the molecular switch controlling anthocyanin accumulation in peach skin and provide a practical tool for marker-assisted selection.",
  "abstract_shown": true,
  "affiliations": [
    "Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China",
    "Key Laboratory of Genetic Resource Evaluation and Application of Horticultural Crops (Fruit), Ministry of Agriculture and Rural Affairs, Zhengzhou 450009, China",
    "Henan Joint International Research Laboratory of Fruit Tree Genomics, Zhengzhou 450009, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Zhenhua Lu"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Lei Pan"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhenyu Huang",
      "orcid": "0000-0002-0341-6824"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Liang Niu"
    },
    {
      "affs": [
        0
      ],
      "name": "Guochao Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenfang Zeng",
      "orcid": "0000-0002-7792-4725"
    },
    {
      "affs": [
        0
      ],
      "name": "Lifeng Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Suke Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Luwei Wang",
      "orcid": "0000-0002-7839-2687"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao Wang",
      "orcid": "0009-0003-6721-429X"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhiqiang Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4642jsmn6cy74.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms272110027",
  "fulltext_url": null,
  "id": "4642jsmn6cy74",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/10027",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-09",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4642jsmn6cy74.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:39Z",
      "native_id": "10.3390/ijms272110027",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/10027"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "A Novel 26-bp Insertion in the PpMYB10.1 Promoter Is Associated with Anthocyanin Deficiency in Peach (Prunus persica) Fruit Skin",
  "title_text": "A Novel 26-bp Insertion in the PpMYB10.1 Promoter Is Associated with Anthocyanin Deficiency in Peach (Prunus persica) Fruit Skin",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:39Z",
  "url": "https://research.draconitic.com/paper/4642jsmn6cy74",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}