{
  "abstract": "Background\n\nAllergic asthma is a heterogeneous inflammatory airway disorder driven by dysregulated immune responses and epithelial-immune crosstalk. Dihydroartemisinin (DHA), an active derivative of artemisinin, exhibits broad anti-inflammatory and immunomodulatory properties; however, its mechanistic role in allergic asthma remains incompletely defined.Methods\n\nNetwork pharmacology was employed to predict potential therapeutic targets and signaling pathways of DHA in asthma. These predictions were validated in a house dust mite (HDM) induced murine asthma model and HDM stimulated human bronchial epithelial (HBE) cells using histopathology, flow cytometry, RT-qPCR, Western blotting, ELISA, and molecular docking.Results\n\nNetwork analysis identified nine hub targets and highlighted the PI3K-AKT pathway as a central regulatory axis. In vivo, DHA markedly attenuated airway inflammation, reduced eosinophil and neutrophil infiltration, and decreased Th2 cytokine production. In HDM-stimulated HBE cells, DHA suppressed pro-inflammatory cytokine expression and downregulated EGFR, MMP9, and PTGS2. Mechanistically, DHA inhibited activation of the PI3K-AKT-mTOR signaling cascade both in vitro and in vivo, consistent with molecular docking predictions demonstrating stable binding of DHA to PI3K related targets.Conclusions\n\nDHA alleviates allergic airway inflammation predominantly through suppression of the PI3K-AKT signaling axis, thereby modulating epithelial inflammatory responses and type 2 immunity. These findings provide mechanistic insight into DHA-mediated immunoregulation and support its potential repositioning as a therapeutic candidate for allergic asthma.",
  "abstract_shown": true,
  "affiliations": [
    "The First Dongguan Affiliated Hospital, College of Medical Technology, Guangdong Medical University, Dongguan, 523808, China. ouyangsd@gdmu.edu.cn.",
    "Dongguan Key Laboratory of Immune Regulation and Prevention of Pulmonary Diseases, Liaobu Hospital of Dongguan City, Dongguan, 523430, China. ouyangsd@gdmu.edu.cn.",
    "Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, Guangdong Medical University, Dongguan, 523808, China. ouyangsd@gdmu.edu.cn.",
    "The First Dongguan Affiliated Hospital, College of Medical Technology, Guangdong Medical University, Dongguan, 523808, China.",
    "Dongguan Key Laboratory of Immune Regulation and Prevention of Pulmonary Diseases, Liaobu Hospital of Dongguan City, Dongguan, 523430, China.",
    "Dongguan Key Laboratory of Immune Regulation and Prevention of Pulmonary Diseases, Liaobu Hospital of Dongguan City, Dongguan, 523430, China. 358581244@qq.com.",
    "Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, Guangdong Medical University, Dongguan, 523808, China. gonghua.huang@gdmu.edu.cn.",
    "The First Dongguan Affiliated Hospital, College of Medical Technology, Guangdong Medical University, Dongguan, 523808, China. huangxueqin@gdmu.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Suidong Ouyang"
    },
    {
      "affs": [
        3
      ],
      "name": "Daichi Chen"
    },
    {
      "affs": [
        3
      ],
      "name": "Hangyu Li"
    },
    {
      "affs": [
        3
      ],
      "name": "Jingyi Deng"
    },
    {
      "affs": [
        3
      ],
      "name": "Yuqia Xie"
    },
    {
      "affs": [
        4
      ],
      "name": "Su Chen"
    },
    {
      "affs": [
        5
      ],
      "name": "Xinlong Lian"
    },
    {
      "affs": [
        6
      ],
      "name": "Gonghua Huang"
    },
    {
      "affs": [
        7
      ],
      "name": "Xueqin Huang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/46ncbdpw0qgd5.bib",
  "cited_by": 0,
  "doi": "10.1186/s12931-026-03956-0",
  "fulltext_url": "https://europepmc.org/articles/PMC13723100",
  "id": "46ncbdpw0qgd5",
  "issns": [
    "1465-9921",
    "1465-993X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42963570",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "2737|Asthma and respiratory diseases"
    ]
  ],
  "openalex": "W7154687079",
  "pdf_url": "https://europepmc.org/articles/PMC13723100?pdf=render",
  "pmcid": "PMC13723100",
  "pmid": "42963570",
  "published": "2026-10-15",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/46ncbdpw0qgd5.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T03:21:43Z",
      "native_id": "MED:42963570",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42963570"
    },
    {
      "first_seen": "2026-11-04T12:21:46Z",
      "native_id": "PMC13723100",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s12931-026-03956-0"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Asthma and respiratory diseases",
      "subfield": "physiology"
    }
  ],
  "title": "Dihydroartemisinin alleviates allergic airway inflammation by suppressing the PI3K-AKT signaling axis: integrated network pharmacology and experimental validation.",
  "title_text": "Dihydroartemisinin alleviates allergic airway inflammation by suppressing the PI3K-AKT signaling axis: integrated network pharmacology and experimental validation.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T12:21:46Z",
  "url": "https://research.draconitic.com/paper/46ncbdpw0qgd5",
  "venue": "Respiratory research",
  "versions": []
}