{
  "abstract": "Amyloid fibril formation is implicated in numerous protein misfolding disorders. Fucoidan, a sulfated polysaccharide primarily derived from brown algae, exhibits diverse biological activities. In this study, we investigated the inhibitory effect of Sargassum horneri fucoidan (S-fucoidan) on insulin amyloid fibrillation in vitro. Initial screening of fucoidans derived from Cladosiphon okamuranus, Kjellmaniella crassifolia, and S. horneri identified S-fucoidan as the most effective inhibitor of insulin fibrillation. Therefore, we characterized the inhibitory effect of S-fucoidan using Thioflavin T fluorescence, transmission electron microscopy, circular dichroism spectroscopy, size-exclusion chromatography, and cell viability assays. Both S-fucoidan and low-molecular-weight S-fucoidan inhibited fibrillation in a concentration-dependent manner. When present from the onset of the reaction, S-fucoidan prevented detectable fibril formation, preserved the initial secondary structure of insulin, and maintained insulin in a monomeric or near-monomeric state. In contrast, addition during nucleation or elongation did not effectively halt ongoing fibrillation. These findings indicate that S-fucoidan predominantly suppresses the early stage of insulin amyloid fibrillation and provide a basis for investigating the molecular mechanisms underlying its anti-fibrillation activity.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Engineering, Graduate School of Sustainability Science, Tottori University, 4-101 Koyamacho-minami, Tottori-city 680-8552, Tottori Prefecture, Japan",
    "Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyamacho-minami, Tottori-city 680-8552, Tottori Prefecture, Japan",
    "Marine Products KIMURAYA Co., Ltd., 3307 Wataricho, Sakaiminato-city 684-0072, Tottori Prefecture, Japan",
    "Department of Chemistry and Biotechnology, Faculty of Engineering, Tottori University, 4-101 Koyamacho-minami, Tottori-city 680-8552, Tottori Prefecture, Japan",
    "Center for Research on Green Sustainable Chemistry, Tottori University, 4-101 Koyamacho-minami, Tottori-city 680-8552, Tottori Prefecture, Japan",
    "Research Center for Utilization of Unused Biological Resources, Tottori University, 4-101 Koyamacho-minami, Tottori-city 680-8550, Tottori Prefecture, Japan"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Noriko Amishiro"
    },
    {
      "affs": [
        1
      ],
      "name": "Asuka Miwa"
    },
    {
      "affs": [
        0
      ],
      "name": "Shiho Kariya"
    },
    {
      "affs": [
        1
      ],
      "name": "Yuka Ito"
    },
    {
      "affs": [
        1
      ],
      "name": "Yukihiro Yasaski"
    },
    {
      "affs": [
        2
      ],
      "name": "Minoru Funakoshi"
    },
    {
      "affs": [
        3,
        4,
        5
      ],
      "name": "Takashi Ohshiro"
    },
    {
      "affs": [
        3,
        4,
        5
      ],
      "name": "Hisashi Yagi",
      "orcid": "0000-0001-6797-8300"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4747kcjzbmxgp.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219516",
  "fulltext_url": null,
  "id": "4747kcjzbmxgp",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9516",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2737|Alzheimer's disease research and treatments"
    ]
  ],
  "openalex": "W7223859971",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-24",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4747kcjzbmxgp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27219516",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9516"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Alzheimer's disease research and treatments",
      "subfield": "physiology"
    }
  ],
  "title": "Sargassum horneri Fucoidan Suppresses the Early-Stage Amyloid Fibrillation of Insulin",
  "title_text": "Sargassum horneri Fucoidan Suppresses the Early-Stage Amyloid Fibrillation of Insulin",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:57Z",
  "url": "https://research.draconitic.com/paper/4747kcjzbmxgp",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}