{
  "abstract": "Objectives: The prevalence of cervicovaginal high-risk human papillomavirus (hrHPV) is high among women with human immunodeficiency virus (WHIV). Many hrHPV tests exist, but genotype-specific comparisons among WHIV are limited. We compared hrHPV detection and diagnostic accuracy using AnyplexTM II HPV 28 and XpertTM HPV among WHIV in Lusaka, Zambia.\n\nMethods: WHIV enrolled in a cervical screening test accuracy study (05/2019-03/2021) were invited for rescreening after 36 months. Trained nurses collected the cervical cytology and biopsy samples. Cytology samples were tested using Anyplex and Xpert. Women with paired and valid hrHPV results were included. We compared hrHPV and genotype-specific prevalence, overall agreement, Cohen kappa (κ), and relative sensitivity and specificity for the detection of high-grade squamous intraepithelial lesions (HSIL).\n\nResults: Among 230 women seen between 05/2022 and 06/2023, hrHPV prevalence was 24% (55/230) using Xpert and 36% (82/230) using Anyplex. Detection of HPV16 was higher with Anyplex (9%, 20/230) than with Xpert (6%, 13/230). Overall agreement was 85% (κ = 0.64; 95% confidence interval [CI] = 0.53-0.74). Genotype-specific agreement ranged from 88% (other hrHPV, κ = 0.66) to 96% (HPV16, κ = 0.70). For the detection of HSIL, the relative sensitivity of Anyplex compared with Xpert was 1.07 (95% CI = 0.94-1.21), and the relative specificity was 0.85 (95% CI = 0.79-0.91).\n\nConclusion: Agreement between Anyplex and Xpert for hrHPV detection was high, but genotype-specific detection differed, with Anyplex detecting HPV16 more frequently than Xpert. While both assays had similar sensitivity, Xpert was more specific for HSIL. The choice of assay should balance the goals of maximizing HSIL detection with minimizing overtreatment.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland",
    "Department of Reproductive, Maternal, Newborn, and Child Health, Centre for Infectious Disease Research in Zambia, Lusaka, Zambia",
    "Molecular Pathology Department, Lancet Laboratories, Johannesburg, South Africa",
    "Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, World Health Organization (IARC/WHO), Lyon, France",
    "Women and Newborn Hospital, University Teaching Hospital, Lusaka, Zambia",
    "Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nathalie Veronica Fernandez Villalobos"
    },
    {
      "affs": [
        1
      ],
      "name": "Misinzo Moono"
    },
    {
      "affs": [
        0
      ],
      "name": "John Andoh"
    },
    {
      "affs": [
        2
      ],
      "name": "Allison Glass"
    },
    {
      "affs": [
        3
      ],
      "name": "Partha Basu"
    },
    {
      "affs": [
        2
      ],
      "name": "Thamsanqa Madliwa"
    },
    {
      "affs": [
        4
      ],
      "name": "Mulindi Mwanahamuntu"
    },
    {
      "affs": [
        0
      ],
      "name": "Eliane Rohner"
    },
    {
      "affs": [
        0
      ],
      "name": "Nicola Low"
    },
    {
      "affs": [
        1,
        5
      ],
      "name": "Albert Manasyan"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Katayoun Taghavi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/484ayszmx1r5y.bib",
  "cited_by": 0,
  "doi": "10.1016/j.ijregi.2026.101027",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13738138/",
  "id": "484ayszmx1r5y",
  "issns": [
    "2772-7076"
  ],
  "landing_url": "https://doi.org/10.1016/j.ijregi.2026.101027",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Report"
    ],
    [
      "openalex",
      "2713|Cervical Cancer and HPV Research"
    ]
  ],
  "openalex": "W7214300732",
  "pdf_url": null,
  "pmcid": "PMC13738138",
  "pmid": "42991648",
  "published": "2026-09-25",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/484ayszmx1r5y.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:23:10Z",
      "native_id": "PMC13738138",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.ijregi.2026.101027"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Cervical Cancer and HPV Research",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Genotype-specific agreement and accuracy of human papillomavirus DNA detection using AnyplexTM II HPV28 and XpertTM HPV among women with HIV in Lusaka, Zambia: a diagnostic accuracy analysis",
  "title_text": "Genotype-specific agreement and accuracy of human papillomavirus DNA detection using AnyplexTM II HPV28 and XpertTM HPV among women with HIV in Lusaka, Zambia: a diagnostic accuracy analysis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:23:10Z",
  "url": "https://research.draconitic.com/paper/484ayszmx1r5y",
  "venue": "IJID Regions",
  "versions": []
}