{
  "abstract": "Lactuca serriola is a wild lettuce species that synthesizes diverse phenolic compounds involved in plant defense and adaptation to environmental stress. Methyl jasmonate (MeJA), a key signaling molecule in plant defense, is known to regulate secondary metabolism by activating the phenylpropanoid pathway. In the present study, genetically stable hairy root cultures of L. serriola were established to investigate MeJA-induced metabolic reprogramming and its effects on phenolic accumulation and antioxidant defense responses. Hairy roots were induced using Agrobacterium rhizogenes strain ATCC 15834 and molecularly confirmed by PCR amplification of the rolC gene. The selected fast-growing hairy root line was treated with 0, 100, and 200 μM MeJA. MeJA significantly affected both growth and secondary metabolism. Although increasing MeJA concentrations reduced fresh and dry biomass, they promoted the accumulation of total phenolics and flavonoids. HPLC-DAD analysis further demonstrated significant increases in several phenolic metabolites, including caffeic acid, chlorogenic acid, p-coumaric acid, rosmarinic acid, and cinnamic acid. MeJA treatment also enhanced the activities of superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase, indicating activation of antioxidant defense systems associated with jasmonate-mediated stress responses and phenylpropanoid metabolism. This study provides the first comprehensive characterization of MeJA-induced phenolic metabolism in L. serriola hairy roots and demonstrates that MeJA effectively reprograms secondary metabolism toward enhanced phenolic biosynthesis. These findings advance our understanding of jasmonate-regulated defense metabolism in hairy roots while supporting the use of this in vitro system as a controlled platform for future studies and potential biotechnological production of specialized metabolites.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran",
    "Division of Biotechnology, Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran",
    "Department of Agrobiotechnology, Institute of Agriculture, RUDN University, Moscow, Russia",
    "Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran",
    "Department of Genetic Engineering, Agricultural Research, Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Maisa Asheri",
      "orcid": "0000-0002-6117-5758"
    },
    {
      "affs": [
        0
      ],
      "name": "Alireza Farokhzad",
      "orcid": "0000-0002-7280-0862"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Mohammadreza Naghavi"
    },
    {
      "affs": [
        3
      ],
      "name": "Raheleh Ghasemzadeh"
    },
    {
      "affs": [
        4
      ],
      "name": "Pejman Azadi"
    },
    {
      "affs": [
        2
      ],
      "name": "Meisam Zargar"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/487f21w06yf21.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0360818",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13734474/",
  "id": "487f21w06yf21",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0360818",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Anatomy"
    ],
    [
      "heading",
      "Antioxidants"
    ],
    [
      "heading",
      "Artificial Gene Amplification and Extension"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Chemical Compounds"
    ],
    [
      "heading",
      "Chemistry"
    ],
    [
      "heading",
      "Enzyme Chemistry"
    ],
    [
      "heading",
      "Enzyme Metabolism"
    ],
    [
      "heading",
      "Enzyme Regulation"
    ],
    [
      "heading",
      "Enzymology"
    ],
    [
      "heading",
      "Hair"
    ],
    [
      "heading",
      "Integumentary System"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Metabolism"
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    [
      "heading",
      "Metabolites"
    ],
    [
      "heading",
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    [
      "heading",
      "Molecular Biology Techniques"
    ],
    [
      "heading",
      "Phenols"
    ],
    [
      "heading",
      "Physical Sciences"
    ],
    [
      "heading",
      "Polymerase Chain Reaction"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "heading",
      "Research and Analysis Methods"
    ],
    [
      "heading",
      "Secondary Metabolism"
    ],
    [
      "plos",
      "/Biology and life sciences/Anatomy/Integumentary system/Hair"
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    [
      "plos",
      "/Biology and life sciences/Biochemistry/Antioxidants"
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      "/Biology and life sciences/Biochemistry/Enzymology/Enzyme chemistry/Enzyme regulation"
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      "plos",
      "/Biology and life sciences/Biochemistry/Metabolism/Metabolites"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Metabolism/Secondary metabolism"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction"
    ],
    [
      "plos",
      "/Medicine and health sciences/Anatomy/Integumentary system/Hair"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Chemical compounds/Phenols"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction"
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  "openalex": null,
  "pdf_url": null,
  "pmcid": "PMC13734474",
  "pmid": "42981316",
  "published": "2026-11-04",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/487f21w06yf21.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T12:20:11Z",
      "native_id": "10.1371/journal.pone.0360818",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:enzymology",
      "subfield": "enzymology-metabolism"
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    {
      "field": "biochemistry",
      "origin": "heading:Molecular Biology",
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  ],
  "title": "Establishment of hairy root cultures of Lactuca serriola and enhancement of phenolic metabolites through methyl jasmonate elicitation",
  "title_text": "Establishment of hairy root cultures of Lactuca serriola and enhancement of phenolic metabolites through methyl jasmonate elicitation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:23:59Z",
  "url": "https://research.draconitic.com/paper/487f21w06yf21",
  "venue": "PLOS One",
  "versions": []
}