{
  "abstract": "This study investigated the effects of temperature on microbial communities, key enzymes, and energy metabolism in carbon fiber fixed-bed anaerobic reactors under psychrophilic (15 °C), mesophilic (37 °C), and thermophilic (55 °C) conditions. Metagenomic and 16S rRNA gene sequencing revealed that methane content peaked at 80% under psychrophilic conditions, despite lower biogas production (7.5 L/d) compared to mesophilic (25 L/d) and thermophilic (30 L/d) regimes. Bacterial diversity was highest at 15 °C, while archaeal richness peaked at 37 °C. Methanothermobacter and Methanosarcina dominated thermophilic methanogenesis, whereas Methanothrix prevailed at lower temperatures. Genes encoding F420-reducing hydrogenase were most abundant at 55 °C, where biogas production was highest, while CO2-reducing methanogenesis genes were well represented at 15 °C, where methane purity reached 80%. ATP synthesis genes shifted from bacteria-associated (37 °C) to archaea-associated (55 °C) dominance. These findings highlight the genetic potential of psychrophilic systems for achieving high methane purity and provide insights for optimizing anaerobic digestion across temperature gradients.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Environment and Quality Test, Chongqing Chemical Industry Vocational College",
    "Key Laboratory for Brewing Microorganism Application and Detection Technology, Luzhou Vocational and Technical College",
    "Chongqing Key Laboratory of Bioresource for Bioenergy, College of Resources and Environment, Southwest University",
    "Chemical Center, Chongqing Shiyi Product Quality Testing Co., Ltd"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xiaoli Pan"
    },
    {
      "affs": [
        1
      ],
      "name": "Hong Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Jiemin Zhu"
    },
    {
      "affs": [
        3
      ],
      "name": "Xugen Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Yongjiang Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jie Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijun Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xixi Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuhua Han"
    },
    {
      "affs": [
        2
      ],
      "name": "Feng Luo"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4a7k59f0w0w7p.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-61884-x",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-61884-x",
  "id": "4a7k59f0w0w7p",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-61884-x",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "2215|Anaerobic Digestion and Biogas Production"
    ]
  ],
  "openalex": "W7170793729",
  "pdf_url": "https://www.nature.com/articles/s41598-026-61884-x_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/4a7k59f0w0w7p.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:42Z",
      "native_id": "310dc25f04cf46919238978ee4a35467",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-61884-x"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:R",
      "subfield": "general"
    }
  ],
  "title": "Temperature drives microbial community shifts and methanogenic pathways in a carbon fiber fixed-bed reactor for anaerobic digestion",
  "title_text": "Temperature drives microbial community shifts and methanogenic pathways in a carbon fiber fixed-bed reactor for anaerobic digestion",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:42Z",
  "url": "https://research.draconitic.com/paper/4a7k59f0w0w7p",
  "venue": "Scientific Reports",
  "versions": []
}