{
  "abstract": "Chicken primordial germ cells (PGCs) are valuable for breeding, germplasm preservation, and genetic modifications. Here, using single-cell transcriptome analysis, we found that three transcriptionally distinct PGC subtypes were exist in chicken embryonic gonads PGCs. We further demonstrated that in vitro-cultured PGCs could integrate into recipient gonads via blood vessel injection. Additionally, the gene-modified PGCs could be re-established and integrated into recipient gonads after the PGCs were cultured for long-term use by a second blood-vessel injection. Together, these results provide evidence for substantial heterogeneity among chicken gonadal PGCs, which were previously considered a homogeneous cell population. This diversity could be leveraged to establish and expand PGCs for long-term in vitro culture from differentiated gonads.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. wangxiaomin@westlake.edu.cn.",
    "Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.",
    "Fudan University, Shanghai, China.",
    "Zhejiang University of Science and Technology School of Information and Electronic Engineering, Hangzhou, China. wangbo@westlake.edu.cn.",
    "Zhejiang Key Laboratory of Biomedical Intelligent Computing Technology, Hangzhou, China. wangbo@westlake.edu.cn.",
    "Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. peiduanqing@westlake.edu.cn.",
    "Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China. peiduanqing@westlake.edu.cn.",
    "Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. zhaochengchen@westlake.edu.cn.",
    "Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China. zhaochengchen@westlake.edu.cn.",
    "Zhejiang Key Laboratory of Biomedical Intelligent Computing Technology, Hangzhou, China. zhaochengchen@westlake.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xiaomin Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Shenghu Zhang"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Kaipeng Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Qianru Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Yihang Yang"
    },
    {
      "affs": [
        1
      ],
      "name": "Xingnan Huang"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhuangfei Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Mengmeng Guo"
    },
    {
      "affs": [
        1
      ],
      "name": "Bo Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Zheting Zhang"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Bo Wang"
    },
    {
      "affs": [
        5,
        6
      ],
      "name": "Duanqing Pei"
    },
    {
      "affs": [
        7,
        8,
        9
      ],
      "name": "Chengchen Zhao",
      "orcid": "0000-0002-8454-6347"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4axfq68nwtsax.bib",
  "cited_by": 0,
  "doi": "10.1007/s00018-026-06331-w",
  "fulltext_url": "https://europepmc.org/articles/PMC13680580",
  "id": "4axfq68nwtsax",
  "issns": [
    "1420-682X",
    "1420-9071"
  ],
  "landing_url": "https://europepmc.org/article/MED/42897262",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ],
    [
      "lcc",
      "QR1-502"
    ],
    [
      "openalex",
      "1311|Animal Genetics and Reproduction"
    ]
  ],
  "openalex": "W7170039282",
  "pdf_url": "https://europepmc.org/articles/PMC13680580?pdf=render",
  "pmcid": "PMC13680580",
  "pmid": "42897262",
  "published": "2026-07-01",
  "publisher": "Springer",
  "ris": "https://research.draconitic.com/paper/4axfq68nwtsax.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:42Z",
      "native_id": "MED:42897262",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42897262"
    },
    {
      "first_seen": "2026-10-30T13:45:50Z",
      "native_id": "e562b89c3c5b4bb691b4dcb09f1a01b9",
      "source": "doaj",
      "url": "https://doi.org/10.1007/s00018-026-06331-w"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Animal Genetics and Reproduction",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.",
  "title_text": "Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:50Z",
  "url": "https://research.draconitic.com/paper/4axfq68nwtsax",
  "venue": "Cellular and molecular life sciences : CMLS",
  "versions": []
}