{
  "abstract": "Adherent-invasive Escherichia coli (AIEC) is frequently isolated from the dysbiotic gut microbiome in inflammatory bowel disease and is implicated in disease progression, highlighting its potential as a therapeutic target. Here we show that iron acquisition is essential for AIEC colonization of the inflamed gut. Mechanistically, four catecholate siderophore receptors act redundantly to support AIEC growth under iron-limited conditions and during colitis. Immunization of colitis-prone Il10-/- mice against catecholate siderophores reduced intestinal AIEC colonization, particularly its association with the mucosa, while largely preserving overall gut microbiome composition. Longitudinal metagenomic profiling identified immunization-associated shifts in Akkermansiaceae and Lactobacillaceae, and experiments in gnotobiotic Il10-/- mice demonstrated that Limosilactobacillus reuteri, a member of the Lactobacillaceae, limited AIEC colonization, whereas Akkermansiaceae exacerbated disease. Our findings establish catecholate siderophore-mediated iron acquisition as a therapeutic target in AIEC and provide proof-of-principle that virulence-targeted immunization can selectively antagonize a disease-associated bacterium while preserving the gut microbiota",
  "abstract_shown": true,
  "affiliations": [
    "University of California, San Diego"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "name": "G. J. Norton"
    },
    {
      "name": "A. M. Dudek"
    },
    {
      "name": "R. R. Gerner"
    },
    {
      "name": "C. Guo"
    },
    {
      "name": "V. Castillo"
    },
    {
      "name": "F. A. Argueta"
    },
    {
      "name": "C. J. Beebout"
    },
    {
      "name": "G. Kanyabi"
    },
    {
      "name": "J. Drushell"
    },
    {
      "name": "G. T. Walker"
    },
    {
      "name": "C.-Y. Hsu"
    },
    {
      "name": "S. Bessho"
    },
    {
      "name": "S.-P. Nuccio"
    },
    {
      "name": "H. Chu"
    },
    {
      "name": "K. Zengler"
    },
    {
      "name": "E. M. Nolan"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Raffatellu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4azbzcbgmt4ec.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.02.756324",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756324v1.full",
  "id": "4azbzcbgmt4ec",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756324v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "1310|Escherichia coli research studies"
    ]
  ],
  "openalex": "W7220411093",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756324v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-02",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4azbzcbgmt4ec.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:07Z",
      "native_id": "10.64898/2026.10.02.756324",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756324v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Targeting catecholate siderophore systems enables precision depletion of gut enterobacteria during colitis",
  "title_text": "Targeting catecholate siderophore systems enables precision depletion of gut enterobacteria during colitis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:07Z",
  "url": "https://research.draconitic.com/paper/4azbzcbgmt4ec",
  "venue": "bioRxiv",
  "versions": []
}