{
  "abstract": "Background: Blood-based biomarkers are transforming Alzheimer's Disease (AD) diagnosis and staging. Recent multi-protein plasma panels accurately identify individuals with advanced Braak pathology, but whether these circulating biomarkers truly reflect the molecular remodeling underlying AD neuropathology remains unclear. Pre-existing datasets could answer such questions, but their reuse requires harmonized protein expression data, standardized sample annotations, and consistent study metadata. Methods: A recently reported seven-protein plasma staging panel was evaluated across multiple post-mortem proteomics brain cohorts using pre-structured datasets on the Tesorai platform. Five datasets with Braak stage data were identified and re-analyzed. A linear model was used to distinguish late (V-VI) from early Braak stage (0-IV). Because phosphorylated tau 217 (p-tau217) and amyloid beta 1-40 (A{beta}40) were not reported for any studies, raw spectra were reprocessed to quantify these proteoforms. Results: Across the cohorts, the plasma-derived biomarker panel consistently discriminated early from late disease despite heterogeneous protein coverage. Reprocessing of raw spectra recovered tau phosphopeptides absent from the original protein summaries, enabling inclusion of p-tau217, while A{beta}40 remained undetected. Together, these findings show that proteins comprising a recently proposed blood-based staging panel are associated with proteomic remodeling in the AD brain across independent cohorts. Conclusions: These findings provide biological validation for a recently proposed blood-based seven-protein staging panel by demonstrating that its constituent biomarkers are associated with disease-stage proteomic changes in AD brain tissue. More broadly, re-mining legacy mass spectrometry data for disease-relevant proteoforms, combined with pre-structured datasets, can accelerate evaluation of emerging blood-based biomarkers against neuropathological and molecular features of disease.",
  "abstract_shown": true,
  "affiliations": [
    "Tesorai"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "C. C. Kim"
    },
    {
      "name": "M. K. Campbell"
    },
    {
      "name": "M. Burq"
    },
    {
      "name": "A. Potocnik"
    },
    {
      "name": "D. Stepec"
    },
    {
      "name": "C. M. Cuoco"
    },
    {
      "name": "Y. Bronevetsky"
    },
    {
      "name": "D. Zafred"
    },
    {
      "name": "J. M. Leung"
    },
    {
      "name": "P. Cimermancic"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4b23s2w6c711m.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.31.748393",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.08.31.748393v1.full",
  "id": "4b23s2w6c711m",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.08.31.748393v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ],
    [
      "openalex",
      "2737|Alzheimer's disease research and treatments"
    ]
  ],
  "openalex": "W7208750704",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.08.31.748393v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-31",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4b23s2w6c711m.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:28Z",
      "native_id": "10.64898/2026.08.31.748393",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.08.31.748393v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "Rapid Mechanistic Bridging of an Alzheimer's Disease Plasma Protein Staging Panel Across Brain Proteomics Cohorts",
  "title_text": "Rapid Mechanistic Bridging of an Alzheimer's Disease Plasma Protein Staging Panel Across Brain Proteomics Cohorts",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:28Z",
  "url": "https://research.draconitic.com/paper/4b23s2w6c711m",
  "venue": "bioRxiv",
  "versions": []
}