{
  "abstract": "Centrosome maturation, the rapid growth of the centrosome before cell division, prepares the cell for spindle assembly. Pericentrin (PCNT), a coiled-coil centrosomal protein, is transported to the centrosome by dynein during its translation, and this co-translational transport promotes maturation. How dynein engages PCNT remains unclear. We show that PCNT residues 1393-1525 are required for the dynein-dynactin interaction. This region shares bulk sequence features with canonical dynein cargo adaptors, and three of five synthetic sequences matching these features fully or partially restored transport of a PCNT variant lacking residues 1393-1525. PCNT is predicted to contact dynein heavy chain at sites used by canonical adaptors, and mutating these sites abolished transport. Purified PCNT activated dynein-dynactin directly without additional factors. These results suggest that PCNT binds and activates dynein through an adaptor-like sequence, with no separate adaptor, and that dynein recognizes cargoes by more diverse mechanisms than previously recognized, including an internal rather than N-terminal binding region.",
  "abstract_shown": true,
  "affiliations": [
    "Syracuse University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "W. Zhang"
    },
    {
      "name": "A. G. Sangster"
    },
    {
      "name": "T. T. Nguyen"
    },
    {
      "name": "J. P. Gillies"
    },
    {
      "name": "S. Melancon"
    },
    {
      "name": "J.-L. Shiu"
    },
    {
      "name": "T. H. Huang"
    },
    {
      "name": "A. G. Iragavarapu"
    },
    {
      "name": "X. Jiang"
    },
    {
      "name": "J. Mia"
    },
    {
      "name": "H. Aydin"
    },
    {
      "name": "M. E. DeSantis"
    },
    {
      "name": "A. M. Moses"
    },
    {
      "affs": [
        0
      ],
      "name": "L.-E. Jao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4cdxrmav0zmn9.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.05.22.727342",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.05.22.727342v2.full",
  "id": "4cdxrmav0zmn9",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.05.22.727342v2",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1307|Microtubule and mitosis dynamics"
    ]
  ],
  "openalex": "W7162306751",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.05.22.727342v2.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-05-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4cdxrmav0zmn9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:27:56Z",
      "native_id": "10.64898/2026.05.22.727342",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.05.22.727342v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Pericentrin contains internal adaptor-like sequences that activate dynein directly",
  "title_text": "Pericentrin contains internal adaptor-like sequences that activate dynein directly",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:56Z",
  "url": "https://research.draconitic.com/paper/4cdxrmav0zmn9",
  "venue": "bioRxiv",
  "versions": []
}