{
  "abstract": "Bacterial ribosome arrest peptides couple translation to secretion and membrane homeostasis, providing an intracellular regulatory feedback system. Although several Sec/YidC-associated arrest peptides have been characterized, how evolutionarily diverse peptides converge on equivalent physiological outcomes remains unclear. Here we identify LudP (Lacrimispora upstream of secDF peptide), a previously unrecognized arrest peptide conserved in the genus Lacrimispora and encoded immediately upstream of secDF. In vitro and in vivo assays show that LudP induces robust Gram-positive-specific translation arrest that is released in a Sec signal-dependent manner. Using systematic mutagenesis and deep mutational scanning, we define a compact 15-residue C-terminal arrest element that governs arrest stability and A-site residue selectivity. Cryo-electron microscopy reveals that a LudP nascent-chain -helix in the exit tunnel remodels the peptidyltransferase center, with Asp133-dependent conformational rearrangements of 23S rRNA, notably U2506, sterically and energetically destabilizing productive A-site aminoacyl-tRNA accommodation. These findings support functional convergent evolution of secretion-coupled arrest peptides across bacterial lineages.",
  "abstract_shown": true,
  "affiliations": [
    "University of Hamburg"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "F. Gersteuer"
    },
    {
      "name": "Y. Harada"
    },
    {
      "name": "K. Fujiwara"
    },
    {
      "name": "H. Safdari"
    },
    {
      "name": "A. Harreither"
    },
    {
      "name": "L. Bock"
    },
    {
      "affs": [
        0
      ],
      "name": "D. N. Wilson"
    },
    {
      "name": "S. N. Chiba"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4ctx5nwc1a29c.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.26.761930",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761930v1.full",
  "id": "4ctx5nwc1a29c",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761930v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ],
    [
      "openalex",
      "1312|RNA and protein synthesis mechanisms"
    ]
  ],
  "openalex": "W7224096740",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761930v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4ctx5nwc1a29c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:30:08Z",
      "native_id": "10.64898/2026.10.26.761930",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761930v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "The LudP arrest peptide stalls translation by remodeling the ribosomal A-site",
  "title_text": "The LudP arrest peptide stalls translation by remodeling the ribosomal A-site",
  "type": "preprint",
  "updated_at": "2026-10-30T12:30:08Z",
  "url": "https://research.draconitic.com/paper/4ctx5nwc1a29c",
  "venue": "bioRxiv",
  "versions": []
}