{
  "abstract": "The defensive chemical system of Pheropsophus occipitalis has long been a research focus on biomimetic catalysis and energy applications, yet the biosynthetic origin of hydrogen peroxide (H2O2) in its defensive glands remains unresolved. This work aimed to decipher the underlying catalytic mechanism and develop a novel biomimetic energy supply strategy. Electron paramagnetic resonance (EPR) spectroscopy and molecular docking were employed to verify the radical-mediated pathway of laccase-catalyzed hydroquinone oxidation. Laccase and catalase were co-immobilized on cerium oxide–titanium dioxide composite supports to enhance catalytic stability and efficiency. A biomimetic microchannel catalyst bed coupled with a pulsed cascade booster system was further constructed, inspired by the compartmentalized anatomy of P. occipitalis. We demonstrate for the first time that laccase catalyzes hydroquinone oxidation to generate H2O2 via radical intermediates, and reveal a triple synergistic catalytic cycle consisting of oxygen regeneration, hydrogen element turnover, and cerium redox shuttle. The immobilized laccase yields a relative H2O2 concentration of 191.79%, corresponding to a 9.2-fold improvement over free laccase. The integrated biomimetic system realizes on-demand in situ generation and utilization of H2O2. This study advances the mechanistic understanding of the P. occipitalis chemical defense system, and provides a novel, low-noise and eco-friendly technical route for the development of portable energy supply systems.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life Science, Anhui University , , ,",
    "State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences , ,",
    "School of Biotechnology, Jiangnan University , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lin Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Yun Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Fanghan Che"
    },
    {
      "affs": [
        0
      ],
      "name": "Xinyi Yao"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiajun Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Weiping Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Ruoshui Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Ruochun Yin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4e6rqsa88mbvs.bib",
  "cited_by": 0,
  "doi": "10.3389/fchem.2026.1831087",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13729060/?tool=EBI",
  "id": "4e6rqsa88mbvs",
  "issns": [
    "2296-2646"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13729060",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "1312|Enzyme Catalysis and Immobilization"
    ]
  ],
  "openalex": "W7223033256",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13729060/pdf/?tool=EBI",
  "pmcid": "PMC13729060",
  "pmid": "42979044",
  "published": "2026-10-20",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/4e6rqsa88mbvs.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:46Z",
      "native_id": "PMC:PMC13729060",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13729060"
    },
    {
      "first_seen": "2026-11-07T12:21:53Z",
      "native_id": "PMC13729060",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fchem.2026.1831087"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Enzyme Catalysis and Immobilization",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Establishment of new energy supply construction inspired by the defensive mechanism of Pheropsophus occipitalis",
  "title_text": "Establishment of new energy supply construction inspired by the defensive mechanism of Pheropsophus occipitalis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:53Z",
  "url": "https://research.draconitic.com/paper/4e6rqsa88mbvs",
  "venue": "Frontiers in chemistry",
  "versions": []
}