{
  "abstract": "Fibronectin (FN) is a multifunctional glycoprotein involved in extracellular matrix remodeling, tumor progression, tissue repair, and host–pathogen interactions. Despite its biological and clinical relevance, FN-binding antibodies derived from non-rodent species remain comparatively less explored. Here, one rabbit was immunized with full-length recombinant human FN, eliciting a robust antibody response confirmed by ELISA and Western blotting. Two rabbit-derived single-chain variable fragment (scFv) phage-display libraries were constructed from peripheral blood mononuclear cells, yielding 5.5 × 107 and 5.0 × 107 transformants for the short- and long-linker formats, respectively. After four rounds of biopanning, phage ELISA confirmed enrichment, and sequence analysis grouped selected clones into five short-linker and four long-linker groups. Seven purified scFvs displayed distinct ELISA binding profiles. Fiber-optic particle plasmon resonance (FOPPR)-based kinetic analysis yielded apparent equilibrium dissociation constants (KD,app) of 11.21–432.06 nM, with FN-L13 showing the lowest KD,app. Analytical size-exclusion chromatography indicated predominantly monomeric scFvs. Five scFvs recognized a high-molecular-weight band consistent with fibronectin in mouse plasma by Western blotting. ELISA showed preferential FN binding, with generally low reactivity toward human serum albumin (HSA) and collagens I, III, and IV, although FN-S1 and FN-L1 showed detectable fibrinogen reactivity. These findings support rabbit-derived FN-binding scFvs as research reagents for future FN-related assay development and mechanistic studies.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate Institute of Biodefense, National Defense Medical University, Taipei 114201, Taiwan",
    "Institute of Preventive Medicine, National Defense Medical University, Taipei 114201, Taiwan",
    "Department of Occupational Medicine and Clinical Toxicology, Taipei Veterans General Hospital, Taipei 112201, Taiwan",
    "Department of Biology and Anatomy, National Defense Medical University, Taipei 114201, Taiwan",
    "Zinexts Life Science Corp., New Taipei City 221416, Taiwan",
    "JW Diagnostics Inc., New Taipei City 235048, Taiwan",
    "Department of New Drug Development, GlycoNex Inc., New Taipei City 221021, Taiwan",
    "Department of R&D, Zinexts Life Science Corp., New Taipei City 221416, Taiwan",
    "School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan",
    "Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan",
    "Navi Bio-Therapeutics Inc., Taipei 10351, Taiwan",
    "Division of Clinical Toxicology, Department of Emergency Medicine, Taichung Veterans General Hospital, Taichung 407219, Taiwan",
    "Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan",
    "iReal Biotechnology Inc., Research and Development Center, New Taipei City 248022, Taiwan",
    "Department of Project Management, GlycoNex Inc., New Taipei City 221021, Taiwan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Yu-Wei Chiang"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Jia-Lin Juang"
    },
    {
      "affs": [
        6
      ],
      "name": "Yi-Yuan Yang"
    },
    {
      "affs": [
        7
      ],
      "name": "Sy-Jye Leu"
    },
    {
      "affs": [
        8,
        9
      ],
      "name": "Jun-Jen Liu"
    },
    {
      "affs": [
        10
      ],
      "name": "Bor-Yu Tsai"
    },
    {
      "affs": [
        11
      ],
      "name": "Yan-Chiao Mao",
      "orcid": "0000-0002-8561-0582"
    },
    {
      "affs": [
        12
      ],
      "name": "Fang-Yi Yen"
    },
    {
      "affs": [
        8
      ],
      "name": "Jia-Yun Chiang"
    },
    {
      "affs": [
        13
      ],
      "name": "Valencia Andriani"
    },
    {
      "affs": [
        14
      ],
      "name": "Priskila Cherisca Thenaka",
      "orcid": "0009-0006-6462-8544"
    },
    {
      "affs": [
        9
      ],
      "name": "Ta-Lun Tan"
    },
    {
      "affs": [
        8,
        9
      ],
      "name": "Ting-Jing Shen",
      "orcid": "0000-0002-1714-0048"
    },
    {
      "affs": [
        0,
        13
      ],
      "name": "Chi-Hsin Lee",
      "orcid": "0000-0002-3412-7400"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4f5gxz4vd3eaj.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27209074",
  "fulltext_url": "https://europepmc.org/articles/PMC13705019",
  "id": "4f5gxz4vd3eaj",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/9074",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2741|Monoclonal and Polyclonal Antibodies Research"
    ]
  ],
  "openalex": "W7221595211",
  "pdf_url": "https://europepmc.org/articles/PMC13705019?pdf=render",
  "pmcid": "PMC13705019",
  "pmid": "42941847",
  "published": "2026-10-13",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4f5gxz4vd3eaj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27209074",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/9074"
    },
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42941847",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42941847"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Monoclonal and Polyclonal Antibodies Research",
      "subfield": "imaging"
    }
  ],
  "title": "Construction and Characterization of Rabbit scFv Antibodies Targeting Fibronectin by Phage Display Technology",
  "title_text": "Construction and Characterization of Rabbit scFv Antibodies Targeting Fibronectin by Phage Display Technology",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/4f5gxz4vd3eaj",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}