{
  "abstract": "Background\n\nReal-time quaking-induced conversion (RT-QuIC) of recombinant prion protein is a biomarker for Creutzfeldt-Jakob disease (CJD). RT-QuIC assesses a sample's seeding activity across multiple wells. We aim to model the test within a binomial framework and describe possible applications.Methods\n\nThis is a single-centre retrospective observational study. CJD and non-CJD cases with cerebrospinal fluid RT-QuIC were enrolled (2014-2018). The probability of a positive event in one well (θ+) for each group was assessed. θ+ can be used to calculate the posterior probability of disease (P_post) or as input parameters for computer simulations.Results\n\nData from 584 sporadic CJD (sCJD) and 226 non-CJD cases were used. Respectively, θ+ was 0.841 (95% CI 0.823 to 0.857) and 0.025 (95% CI 0.016 to 0.040). The distribution of RT-QuIC results did not differ between all sCJD samples and definite sCJD cases and across groups with different alleles at codon 129 of the PRNP gene. P_post was calculated for each result. The distribution of results did not differ significantly across years in def/prob-sCJD and non-CJD.Discussion\n\nRT-QuIC results distribution and θ+ may be used for intra-laboratory quality control and P_post determination. Future studies should explore the utility of θ+ to increase diagnostic accuracy and inter-laboratory comparison.",
  "abstract_shown": true,
  "affiliations": [
    "Neurology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.",
    "National TSE Reference Centre, Department of Neurology, University of Göttingen, Göttingen, Germany.",
    "Vita-Salute San Raffaele University, Milan, Italy.",
    "Neuroimaging Research Unit, Division of Neuroscience, IRCCS San Raffaele Scientific Institute, Milan, Italy.",
    "Neurophysiology Service, IRCCS San Raffaele Scientific Institute, Milan, Italy.",
    "Neurorehabilitation Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.",
    "German Center for Neurodegenerative Diseases (DZNE), University of Göttingen, Göttingen, Germany."
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Matteo Zavarella",
      "orcid": "0009-0007-8290-5856"
    },
    {
      "affs": [
        1
      ],
      "name": "Peter Hermann",
      "orcid": "0000-0003-1889-8325"
    },
    {
      "affs": [
        1
      ],
      "name": "Susana da Silva Correia"
    },
    {
      "affs": [
        1
      ],
      "name": "Sezgi Canaslan Eyyuboglu"
    },
    {
      "affs": [
        1
      ],
      "name": "Matthias Schmitz",
      "orcid": "0000-0002-5502-681X"
    },
    {
      "affs": [
        0,
        2,
        3,
        4,
        5
      ],
      "name": "Massimo Filippi",
      "orcid": "0000-0002-5485-0479"
    },
    {
      "affs": [
        1,
        6
      ],
      "name": "Inga Zerr"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4fqmcrfrpb36z.bib",
  "cited_by": 0,
  "doi": "10.1136/bmjno-2026-001863",
  "fulltext_url": "https://europepmc.org/articles/PMC13723793",
  "id": "4fqmcrfrpb36z",
  "issns": [
    "2632-6140"
  ],
  "landing_url": "https://europepmc.org/article/MED/42968878",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "1506"
    ],
    [
      "heading",
      "Short Report"
    ],
    [
      "openalex",
      "1312|Prion Diseases and Protein Misfolding"
    ]
  ],
  "openalex": "W7224088617",
  "pdf_url": "https://europepmc.org/articles/PMC13723793?pdf=render",
  "pmcid": "PMC13723793",
  "pmid": "42968878",
  "published": "2026-10-28",
  "publisher": "BMJ Publishing Group",
  "ris": "https://research.draconitic.com/paper/4fqmcrfrpb36z.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:53Z",
      "native_id": "MED:42968878",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42968878"
    },
    {
      "first_seen": "2026-11-04T12:22:22Z",
      "native_id": "PMC13723793",
      "source": "pmc",
      "url": "https://doi.org/10.1136/bmjno-2026-001863"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Prion Diseases and Protein Misfolding",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Value of positive RT-QuIC replicate number in sporadic Creutzfeldt-Jakob disease.",
  "title_text": "Value of positive RT-QuIC replicate number in sporadic Creutzfeldt-Jakob disease.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:32Z",
  "url": "https://research.draconitic.com/paper/4fqmcrfrpb36z",
  "venue": "BMJ neurology open",
  "versions": []
}