{
  "abstract": "Many proteins required for embryogenesis are expressed in multiple tissues and across developmental time, often with tissue- and stage-specific roles. To resolve where and when proteins function, we developed a tissue-targeted auxin-inducible degradation toolkit for C. elegans embryogenesis. We generated a plasmid set compatible with single-copy insertion or high-copy extrachromosomal array generation that employs tissue-specific promoters (active in the germline, intestine, muscle, pharynx, neuroblasts, neurons, sensory neurons, epidermis, and pan soma) to drive an operon expressing an optimized auxin-dependent plant F-box factor TIR1 and, in most cases, a fluorescent histone. Using a ubiquitous nuclear envelope marker as a test substrate, we identified single-copy insertions and arrays that exhibit efficient auxin-dependent, tissue-specific target degradation. We also establish a workflow that ensures all functionally important isoforms of a target are tagged and that degradation reproduces the loss-of-function phenotype prior to tissue-specific analysis. Applying the toolkit to the broadly expressed Rho kinase LET-502, we find that degradation in the epidermis, but not in other embryonic tissues, disrupts elongation. Unexpectedly, temporal analysis revealed that LET-502 is required for late as well as early elongation.",
  "abstract_shown": true,
  "affiliations": [
    "University of California San Diego"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "N. Varshney"
    },
    {
      "name": "R. A. Green"
    },
    {
      "name": "A. O. Desai"
    },
    {
      "name": "S. Moghareh"
    },
    {
      "name": "D. J. Green"
    },
    {
      "name": "A. Desai"
    },
    {
      "affs": [
        0
      ],
      "name": "K. Oegema"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4fzrvcsaespqj.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.07.757239",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.07.757239v1.full",
  "id": "4fzrvcsaespqj",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.07.757239v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "developmental biology"
    ],
    [
      "openalex",
      "1312|Developmental Biology and Gene Regulation"
    ]
  ],
  "openalex": "W7221144065",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.07.757239v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4fzrvcsaespqj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:46Z",
      "native_id": "10.64898/2026.10.07.757239",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.07.757239v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:developmental biology",
      "subfield": "developmental-biology"
    }
  ],
  "title": "A tissue-targeted auxin-inducible degradation toolkit for C. elegans embryogenesis",
  "title_text": "A tissue-targeted auxin-inducible degradation toolkit for C. elegans embryogenesis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:46Z",
  "url": "https://research.draconitic.com/paper/4fzrvcsaespqj",
  "venue": "bioRxiv",
  "versions": []
}