{
  "abstract": "Some bacteriophages encode lysis proteins that inhibit essential bacterial processes, and elucidating their mechanisms is valuable for developing antibacterial strategies against drug-resistant pathogens. We determined the cryo-EM structure of the complex between the essential Escherichia coli lipid II flippase MurJ and the phage lysis protein SglPP7. Structural, mutational, and molecular dynamics (MD) simulation analyses revealed that SglPP7 binds along the intramembrane cleft of MurJ and inserts its C-terminal tail into the internal cavity, stabilizing MurJ in an outward-facing conformation. Structural comparison with LysM (SglM) and SglCJ3 showed that these sequence-divergent Sgl proteins stabilize MurJ in the outward-facing conformation through distinct binding modes, consistent with the possibility of convergent evolution toward MurJ inhibition.\n\nBacteriophages encode lysis proteins that inhibit essential bacterial processes. This study solves the cryo-EM structure of the complex between the essential E. coli lipid II flippase MurJ and a phage lysis protein SglPP7, demonstrating that SglPP7 locks MurJ in an outward facing conformation.",
  "abstract_shown": true,
  "affiliations": [
    "Nara Institute of Science and Technology, Ikoma, Nara, Japan",
    "Diffraction and Scattering Division, Japan Synchrotron Radiation Research Institute, Sayo, Hyogo, Japan"
  ],
  "article_type": "Short Reports",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kaito Hosoda"
    },
    {
      "affs": [
        0
      ],
      "name": "Hidetaka Kohga"
    },
    {
      "affs": [
        0
      ],
      "name": "Sixian Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Hiroyuki Tanaka"
    },
    {
      "affs": [
        1
      ],
      "name": "Hideki Shigematsu"
    },
    {
      "affs": [
        0
      ],
      "name": "Ryoji Miyazaki"
    },
    {
      "affs": [
        0
      ],
      "name": "Min Fey Chek"
    },
    {
      "affs": [
        0
      ],
      "name": "Tomoya Tsukazaki",
      "orcid": "0000-0002-6386-723X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4g1adf3dhmeb5.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pbio.3004039",
  "fulltext_url": "https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3004039",
  "id": "4g1adf3dhmeb5",
  "issns": [
    "1545-7885",
    "1544-9173"
  ],
  "landing_url": "https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3004039",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Animal Studies"
    ],
    [
      "heading",
      "Bacteria"
    ],
    [
      "heading",
      "Bacterial Pathogens"
    ],
    [
      "heading",
      "Bacteriophages"
    ],
    [
      "heading",
      "Biochemical Simulations"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Biology and life sciences"
    ],
    [
      "heading",
      "Computational Biology"
    ],
    [
      "heading",
      "DNA construction"
    ],
    [
      "heading",
      "Electron Cryo-Microscopy"
    ],
    [
      "heading",
      "Electron Microscopy"
    ],
    [
      "heading",
      "Enterobacteriaceae"
    ],
    [
      "heading",
      "Escherichia"
    ],
    [
      "heading",
      "Escherichia Coli"
    ],
    [
      "heading",
      "Experimental Organism Systems"
    ],
    [
      "heading",
      "Gut Bacteria"
    ],
    [
      "heading",
      "Lipid Structure"
    ],
    [
      "heading",
      "Lipids"
    ],
    [
      "heading",
      "Lysis (Medicine)"
    ],
    [
      "heading",
      "Medical Microbiology"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Microbial Pathogens"
    ],
    [
      "heading",
      "Microbiology"
    ],
    [
      "heading",
      "Microscopy"
    ],
    [
      "heading",
      "Model Organisms"
    ],
    [
      "heading",
      "Molecular biology"
    ],
    [
      "heading",
      "Molecular biology techniques"
    ],
    [
      "heading",
      "Organisms"
    ],
    [
      "heading",
      "Pathogens"
    ],
    [
      "heading",
      "Pathology and Laboratory Medicine"
    ],
    [
      "heading",
      "Physiological Processes"
    ],
    [
      "heading",
      "Physiology"
    ],
    [
      "heading",
      "Plasmid Construction"
    ],
    [
      "heading",
      "Prokaryotic Models"
    ],
    [
      "heading",
      "Research and Analysis Methods"
    ],
    [
      "heading",
      "Research and analysis methods"
    ],
    [
      "heading",
      "Short Reports"
    ],
    [
      "heading",
      "Tissue Repair"
    ],
    [
      "heading",
      "Viruses"
    ],
    [
      "openalex",
      "1311|Bacterial Genetics and Biotechnology"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Biochemical simulations"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Lipids"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Lipids/Lipid structure"
    ],
    [
      "plos",
      "/Biology and life sciences/Computational biology/Biochemical simulations"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Bacterial pathogens/Escherichia coli"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/DNA construction/Plasmid construction"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Bacteria/Enterobacteriaceae/Escherichia/Escherichia coli"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Bacteria/Gut bacteria/Escherichia/Escherichia coli"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Viruses/Bacteriophages"
    ],
    [
      "plos",
      "/Biology and life sciences/Physiology/Physiological processes/Tissue repair/Lysis (medicine)"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Bacterial pathogens/Escherichia coli"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Escherichia coli"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Prokaryotic models/Escherichia coli"
    ],
    [
      "plos",
      "/Research and analysis methods/Microscopy/Electron microscopy/Electron cryo-microscopy"
    ],
    [
      "plos",
      "/Research and analysis methods/Model organisms/Escherichia coli"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/DNA construction/Plasmid construction"
    ]
  ],
  "openalex": "W7223917427",
  "pdf_url": "https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3004039&type=printable",
  "pmcid": "PMC13712586",
  "pmid": "42945156",
  "published": "2026-10-27",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/4g1adf3dhmeb5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:17Z",
      "native_id": "10.1371/journal.pbio.3004039",
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      "url": "https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3004039"
    },
    {
      "first_seen": "2026-10-31T12:23:08Z",
      "native_id": "PMC13712586",
      "source": "pmc",
      "url": "https://doi.org/10.1371/journal.pbio.3004039"
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  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:molecular biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:computational biology",
      "subfield": "bioinformatics"
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    {
      "field": "biology",
      "origin": "plos:microbiology",
      "subfield": "microbiology"
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    {
      "field": "medicine",
      "origin": "heading:Physiology",
      "subfield": "physiology"
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  ],
  "title": "Structural basis for inhibition of the lipid flippase MurJ by phage lysis protein SglPP7 suggests convergent evolution with other Sgl peptides",
  "title_text": "Structural basis for inhibition of the lipid flippase MurJ by phage lysis protein SglPP7 suggests convergent evolution with other Sgl peptides",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:23:08Z",
  "url": "https://research.draconitic.com/paper/4g1adf3dhmeb5",
  "venue": "PLOS Biology",
  "versions": []
}