{
  "abstract": "Two-photon excitation (2PE) microscopy reaches deep into tissue but remains diffraction-limited, and existing 2PE-STED and 2PE-RESOLFT schemes still rely on single-photon depletion or photoswitching. Here we present 2P-RESOLFT microscopy, in which excitation and reversible photoswitching are both two-photon-driven. Using the positive photoswitchable protein Padron2, we achieved 205-nm lateral resolution and resolved previously unreported membrane protrusions of {beta} cells inside pancreatic pseudoislets.",
  "abstract_shown": true,
  "affiliations": [
    "SANKEN, The University of Osaka"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "R. Ozaki-Noma"
    },
    {
      "name": "H. Ishii"
    },
    {
      "name": "T. Wazawa"
    },
    {
      "name": "J. Sakamoto"
    },
    {
      "name": "Y. Kozawa"
    },
    {
      "name": "Y. Kato"
    },
    {
      "name": "E. Mukai"
    },
    {
      "name": "T. Nemoto"
    },
    {
      "affs": [
        0
      ],
      "name": "T. Nagai"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4g1pck340jpf6.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.13.751305",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751305v1.full",
  "id": "4g1pck340jpf6",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751305v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biophysics"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7213638603",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751305v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4g1pck340jpf6.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:23:13Z",
      "native_id": "10.64898/2026.09.13.751305",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751305v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biophysics",
      "subfield": "biophysics"
    }
  ],
  "title": "Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue",
  "title_text": "Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:13Z",
  "url": "https://research.draconitic.com/paper/4g1pck340jpf6",
  "venue": "bioRxiv",
  "versions": []
}