{
  "abstract": "The development of the mammalian cerebral cortex follows a tightly regulated dynamic molecular program, the critical stages and key molecular players of which remain to be fully elucidated. Detailed visualization of these processes, along with functional genomic studies, will significantly advance our understanding of neurogenesis and its associated human disorders. Such developmental aberrations often entail irreversible changes that lead to congenital malformations of varying severity. In utero electroporation (IUE) has emerged as a widely utilized in vivo transfection and imaging technique. When coupled with advanced technologies such as genome editing and systems for spatiotemporal and tissue-specific gene expression, IUE offers unprecedented opportunities to dissect the molecular mechanisms underlying embryonic cortical development both under normal conditions and in various pathological processes. This review highlights the technical utility of IUE and provides examples of experimental designs used to investigate stages of neurogenesis. Particular attention has been paid to studying the cell cycle using thymidine analogues, the differentiation of neuronal progenitor cells, and axonal guidance.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Neuroscience, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, Russia",
    "Department of Genetics and Life Sciences, Sirius University of Science and Technology, 1 Olympic Ave, 354340 Sirius Federal Territory, Russia",
    "Institute of Theoretical and Experimental Biophysics of Russian Academy of Sciences, 142290 Pushchino, Russia"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ermoshina Anastasiia"
    },
    {
      "affs": [
        0
      ],
      "name": "Okhalnikov Alexandr"
    },
    {
      "affs": [
        0
      ],
      "name": "Kolbasov Pavel"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Dubrovina Assol’"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Tarabykin Victor"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Gavrish Maria"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4gkcvyrmh6x49.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219760",
  "fulltext_url": null,
  "id": "4gkcvyrmh6x49",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9760",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4gkcvyrmh6x49.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:54Z",
      "native_id": "10.3390/ijms27219760",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9760"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Editing the Embryonic Brain: How In Utero Electroporation Reveals the Secrets of Cortical Development",
  "title_text": "Editing the Embryonic Brain: How In Utero Electroporation Reveals the Secrets of Cortical Development",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T00:20:52Z",
  "url": "https://research.draconitic.com/paper/4gkcvyrmh6x49",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}