{
  "abstract": "Ruxolitinib is a Janus kinase inhibitor effective against graft-versus-host disease and hemophagocytic lymphohistiocytosis. However, clinical management is challenged by potential drug-drug interactions and adverse events. Although therapeutic drug monitoring (TDM) can optimize drug safety and efficacy, real-world pharmacokinetic data are limited. To address this gap, we established a simple and sensitive LC-MS/MS method with a total run time of 8.1 min. The method demonstrated linearity over a range of 0.5-100 ng/mL. The intra- and inter-day precision and accuracy met the acceptance criteria. The method was successfully applied to 8 pediatric and 10 adult patients. Measurable concentrations were confirmed in infants (<2 years). The observed trough concentrations varied widely, ranging from 0.69 to 22.2 ng/mL in pediatric patients and <0.5 to 80.6 ng/mL in adult patients. Notably, an intraindividual comparison in one adult case revealed that posaconazole co-administration increased the trough concentration by approximately 4-fold and the area under the plasma concentration-time curve by approximately 2-fold, compared with the period without azoles. In conclusion, we established a validated LC-MS/MS method that was applicable to the measurement of ruxolitinib concentrations in pediatric and adult patients in this single-center study. This method may contribute to the accumulation of pharmacokinetic data for future evaluation of TDM strategies.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "Atsushi Yamaguchi"
    },
    {
      "name": "Yuki Tazawa"
    },
    {
      "name": "Shunsuke Nashimoto"
    },
    {
      "name": "Masahiro Ueki"
    },
    {
      "name": "Shinsuke Hirabayashi"
    },
    {
      "name": "Souichi Shiratori"
    },
    {
      "name": "Fuyo Takeda"
    },
    {
      "name": "Keisuke Kagami"
    },
    {
      "name": "Atsushi Manabe"
    },
    {
      "name": "Takanori Teshima"
    },
    {
      "name": "Mitsuru Sugawara"
    },
    {
      "name": "Yoh Takekuma"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4gybcvfwdagm3.bib",
  "cited_by": 0,
  "doi": "10.1248/bpb.b26-00243",
  "fulltext_url": "https://www.jstage.jst.go.jp/article/bpb/49/9/49_b26-00243/_article",
  "id": "4gybcvfwdagm3",
  "issns": [
    "1347-5215",
    "0918-6158"
  ],
  "landing_url": "https://www.jstage.jst.go.jp/article/bpb/49/9/49_b26-00243/_article",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "jstage-journal",
      "medicine/pharmacology-toxicology"
    ],
    [
      "openalex",
      "1602|Analytical Methods in Pharmaceuticals"
    ]
  ],
  "openalex": "W7213528017",
  "pdf_url": "https://www.jstage.jst.go.jp/article/bpb/49/9/49_b26-00243/_pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-18",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4gybcvfwdagm3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:32Z",
      "native_id": "10.1248/bpb.b26-00243",
      "source": "jstage",
      "url": "https://www.jstage.jst.go.jp/article/bpb/49/9/49_b26-00243/_article"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1602|Analytical Methods in Pharmaceuticals",
      "subfield": "analytical"
    }
  ],
  "title": "Simple LC-MS/MS Method for Ruxolitinib in Human Plasma and Its Clinical Application in Pediatric and Adult Patients",
  "title_text": "Simple LC-MS/MS Method for Ruxolitinib in Human Plasma and Its Clinical Application in Pediatric and Adult Patients",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:32Z",
  "url": "https://research.draconitic.com/paper/4gybcvfwdagm3",
  "venue": "Biological and Pharmaceutical Bulletin",
  "versions": []
}