{
  "abstract": "Early detection is critical for improving survival in colorectal carcinoma (CRC), underscoring the need for reliable early-stage biomarkers. Plasma protein biomarkers may support the detection of early-stage CRC. In this study, we profiled plasma from 24 patients with stages I–III CRC and 10 non-tumor participants, together with paired tumor and distant-normal tissues from the patients with CRC, to identify proteins preferentially elevated in stage I disease. Seven plasma proteins had higher abundance in stage I CRC than in stages II and III CRC and non-tumor participants; three of these proteins, HSPA8, ALDOA, and PDLIM1, were also elevated in stage I tumor tissues. In the discovery comparison of stage I CRC with non-tumor participants, HSPA8 alone achieved an internally cross-validated random forest classification accuracy of 88.9%, matching the three-protein model. Tissue upregulation was further supported by Western blotting. ELISA assessment included 32 participants with matched proteomic measurements and was subsequently expanded to 108 participants. The HSPA8-score was calculated by dividing the dilution-corrected HSPA8 concentration by the total protein amount in 10 μL of plasma. In the expanded cohort, random forest classification using this score achieved internally cross-validated accuracies of 74.47% for stage I versus stages II–IV CRC and 56.67% for stage I CRC versus non-tumor participants. The corresponding exploratory reference cutoffs were 0.066 and 0.029. These findings identify HSPA8 as a potential plasma protein biomarker for stage I CRC detection.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300350, China",
    "Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China",
    "Department of Colorectal Surgery, Tianjin Union Medical Center, Tianjin Institute of Coloproctology, Tianjin 300121, China",
    "Tianjin Union Medical Center, Nankai University, Tianjin 300071, China",
    "Haihe Laboratory of Cell Ecosystem, Tianjin 300020, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Guoxing Yin"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhifei Chen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Guofeng Feng",
      "orcid": "0009-0008-8446-8140"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiangtao Lu"
    },
    {
      "affs": [
        2
      ],
      "name": "Chenmeng Jiao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhengmao Zhu"
    },
    {
      "affs": [
        0,
        1,
        3,
        4
      ],
      "name": "Lin Liu"
    },
    {
      "affs": [
        2
      ],
      "name": "Chen Xu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4kk2qqbvsc39j.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219630",
  "fulltext_url": null,
  "id": "4kk2qqbvsc39j",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9630",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7224098934",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4kk2qqbvsc39j.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27219630",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9630"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Identification of Novel Plasma Protein Biomarkers for Early Clinical Stage Colorectal Carcinoma by High-Throughput Proteomics",
  "title_text": "Identification of Novel Plasma Protein Biomarkers for Early Clinical Stage Colorectal Carcinoma by High-Throughput Proteomics",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:57Z",
  "url": "https://research.draconitic.com/paper/4kk2qqbvsc39j",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}