{
  "abstract": "Background: The interplay between platelets and fibrinogen is the cornerstone of thrombus formation. Integrin alphaIIbbeta3 is the main platelet adhesion receptor for fibrinogen and mediates an outside-in signal upon ligand binding that reinforces platelet activation. In addition, FcgammaRIIA and glycoprotein VI (GPVI) contribute to platelet activation on fibrinogen, thereby participating in thrombus growth and stability. To date, the relative importance of these two immunoreceptor tyrosine-based activation motif-bearing receptors in these processes remains unknown. Objective: The aim of this study was to evaluate the relative contributions of FcgammaRIIA and GPVI to platelet activation on fibrinogen and subsequent thrombus growth and stability. Methods: We evaluated human and mouse platelet adhesion to fibrinogen in static assays and a flow-based approach to evaluate the contribution of FcgammaRIIA and GPVI to thrombus growth and stability. Results: We first confirmed that integrin alphaIIbbeta3 is the key receptor supporting platelet adhesion and spreading on fibrinogen. Using human platelets treated with pharmacological blocking agents and transgenic mouse platelets expressing human receptors, data indicate that GPVI, but not FcgammaRIIA, plays a prominent role in platelet activation on fibrinogen. Moreover, using a flow-based assay, we observed that blockade of GPVI with 1G5, but not FcgammaRIIA with IV.3, prevents thrombus growth. Finally, we observed that 1G5, but not IV.3, promotes the disaggregation of thrombi formed on collagen in vitro. Conclusion: This study provides evidence that GPVI, but not FcgammaRIIA, induces platelet activation and spreading on fibrinogen, and promotes thrombus buildup and stability.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Muhammad Usman Ahmed"
    },
    {
      "name": "Nicolas Receveur"
    },
    {
      "name": "Emily Janus-Bell"
    },
    {
      "name": "Clarisse Mouriaux"
    },
    {
      "name": "Christian Gachet"
    },
    {
      "name": "Martine Jandrot-Perrus"
    },
    {
      "name": "Beatrice Hechler"
    },
    {
      "name": "Elizabeth E. Gardiner"
    },
    {
      "name": "Pierre Mangin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4m3ektmh4jw3b.bib",
  "cited_by": 25,
  "doi": "10.1002/rth2.12551",
  "fulltext_url": null,
  "id": "4m3ektmh4jw3b",
  "issns": [
    "2475-0379",
    "2475-0379"
  ],
  "landing_url": "https://univoak.hal.science/hal-05254033v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.mhep"
    ],
    [
      "hal",
      "2.sdv.mhep.hem"
    ],
    [
      "openalex",
      "2720|Platelet Disorders and Treatments"
    ]
  ],
  "openalex": "W3182544687",
  "pdf_url": "https://univoak.hal.science/hal-05254033/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-14",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/4m3ektmh4jw3b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "hal-05254033",
      "source": "hal",
      "url": "https://univoak.hal.science/hal-05254033v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    },
    {
      "field": "medicine",
      "origin": "hal:1.sdv.mhep",
      "subfield": "general"
    }
  ],
  "title": "Respective roles of Glycoprotein VI and FcgammaRIIA in the regulation of alphaIIbbeta3-mediated platelet activation to fibrinogen, thrombus buildup, and stability",
  "title_text": "Respective roles of Glycoprotein VI and FcgammaRIIA in the regulation of alphaIIbbeta3-mediated platelet activation to fibrinogen, thrombus buildup, and stability",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/4m3ektmh4jw3b",
  "venue": "Research and Practice in Thrombosis and Haemostasis",
  "versions": []
}