{
  "abstract": "Cryopreservation of ram spermatozoa is essential for genetic improvement and germplasm conservation but remains limited by cryo-induced post-thaw deterioration. Conventional egg-yolkbased (EY) extenders suffer from biological variability and biosecurity concerns, motivating the development of chemically defined alternatives. This in vitro study compared a commercial liposome-based extender (LB; OptiXcell®, IMV Technologies) with an EY extender for ram semen cryopreservation using a split-ejaculate design across two independent experiments (42 ejaculates; contrasting management and seasonal conditions). Post-thaw quality was assessed by computer-assisted sperm analysis (CASA; total and progressive motility, VAP, VCL, VSL), flow cytometry (plasma membrane integrity, acrosome integrity, mitochondrial membrane potential), and the hypo-osmotic swelling test (HOST). LB-cryopreserved semen showed higher post-thaw total motility (Experiment I: 34.8 ± 4.2% vs 25.1 ± 3.5%; Experiment II: 40.8 ± 4.1% vs 34.3 ± 4.1%) and progressive motility, with velocity parameters significantly improved. A greater proportion of spermatozoa with intact plasma membranes (Experiment I: 41.9 vs 27.4%; Experiment II: 51.5 vs 37.0%) and preserved acrosomes was detected in LB-treated samples. Mitochondrial membrane potential was higher with LB in Experiment II only (73.5 ± 4.2% vs 59.3 ± 4.2%), reflecting the context-dependent sensitivity of this endpoint. No difference was detected by HOST. Despite inter-individual variability, 94% of ejaculates exhibited higher plasma membrane integrity and two-thirds showed higher total motility with LB. These findings indicate that chemically defined liposome-based extenders improve multiple in vitro indicators of ram sperm function, whilst in vivo fertility trials remain necessary to confirm reproductive relevance.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Elodie Mazenod"
    },
    {
      "name": "Loris Commin"
    },
    {
      "name": "Florine Bontemps"
    },
    {
      "name": "Lionel Boyer"
    },
    {
      "name": "Samuel Buff"
    },
    {
      "name": "Elodie Pillet-Michelland"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4nqx744wt034k.bib",
  "cited_by": 1,
  "doi": "10.1016/j.anireprosci.2026.108238",
  "fulltext_url": null,
  "id": "4nqx744wt034k",
  "issns": [
    "0378-4320",
    "1873-2232"
  ],
  "landing_url": "https://hal.science/hal-05797603v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7161160109",
  "pdf_url": "https://hal.science/hal-05797603/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-05-14",
  "publisher": "Elsevier [1977-....]",
  "ris": "https://research.draconitic.com/paper/4nqx744wt034k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "hal-05797603",
      "source": "hal",
      "url": "https://hal.science/hal-05797603v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Liposome‑based extender consistently improves post-thaw semen quality in rams",
  "title_text": "Liposome‑based extender consistently improves post-thaw semen quality in rams",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/4nqx744wt034k",
  "venue": "Animal Reproduction Science",
  "versions": []
}