{
  "abstract": "Cells preserve genome stability while occasionally generating genetic diversity through mutations and chromosomal rearrangements, but how these opposing outcomes are balanced remains unclear. Because late-replicating regions evolve more rapidly, we investigated the consequences of reduced origin licensing and lengthened S phase in yeast. Under these conditions, cells progress slowly through S phase without checkpoint activation, and enter mitosis with under-replicated DNA. We show that mitotic cyclin-dependent kinase (M-CDK) phosphorylates DNA polymerase α (Pol α), displacing it from replication forks and triggering fork collapse at mitotic entry. The resulting DNA damage response provides a window for mitotic DNA synthesis (MiDAS), enabling completion of replication through error-prone, recombination-dependent mechanisms resembling break-induced replication (BIR) and Fork Stalling & Template Switching (FoSTeS). These findings identify Pol α as a critical M-CDK target that promotes the transition from canonical replication to recombination-driven DNA synthesis when cells enter mitosis with incompletely-duplicated chromosomes. This mechanism offers a potential explanation for the accelerated evolution of late-replicating genomic regions.",
  "abstract_shown": true,
  "affiliations": [
    "IGMM, CNRS, Univ Montpellier, Montpellier, France",
    "Present Address: Dortmund Life Science Center, TU Dortmund, Dortmund, Germany",
    "Present Address: IDgenX, Montpellier, France",
    "MDC, BioCampus, IGMM, CNRS, Univ Montpellier, Montpellier, France",
    "SERANAD, IGMM, CNRS, Univ Montpellier, Montpellier, France",
    "BCM, Univ. Montpellier, CNRS, INSERM, Montpellier, France",
    "IGF, Univ. Montpellier, CNRS, INSERM, Montpellier, France",
    "Dept Biochemistry & Biophysics, UCSF, San Francisco, CA USA"
  ],
  "article_type": "research article",
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  "author_total": 16,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Juan de Dios Barba Tena",
      "orcid": "0000-0002-3562-8071"
    },
    {
      "affs": [
        0
      ],
      "name": "Riccardo Montecchi"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Maxime Lambert",
      "orcid": "0000-0001-7572-5696"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Thomas Defrance",
      "orcid": "0009-0003-7182-4517"
    },
    {
      "affs": [
        0
      ],
      "name": "Elisabeth Gueydon"
    },
    {
      "affs": [
        0
      ],
      "name": "Julie Petit"
    },
    {
      "affs": [
        3
      ],
      "name": "Marjorie Drac",
      "orcid": "0000-0003-3236-5015"
    },
    {
      "affs": [
        0
      ],
      "name": "Noémie Legoff",
      "orcid": "0009-0007-6828-1768"
    },
    {
      "affs": [
        0
      ],
      "name": "Michela Galli"
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    {
      "affs": [
        0
      ],
      "name": "Isabelle Goiffon",
      "orcid": "0009-0000-8681-7916"
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    {
      "affs": [
        4
      ],
      "name": "Thierry Gostan",
      "orcid": "0000-0002-1087-8015"
    },
    {
      "affs": [
        5
      ],
      "name": "Khadija El Koulali"
    },
    {
      "affs": [
        6
      ],
      "name": "Serge Urbach",
      "orcid": "0000-0001-8663-2006"
    },
    {
      "affs": [
        7
      ],
      "name": "David Toczyski",
      "orcid": "0000-0001-5924-0365"
    },
    {
      "affs": [
        0
      ],
      "name": "Nicolas Talarek",
      "orcid": "0000-0003-2804-7372"
    },
    {
      "affs": [
        0
      ],
      "name": "Etienne Schwob",
      "orcid": "0000-0002-9369-6419"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4pamt7h9qerrg.bib",
  "cited_by": 0,
  "doi": "10.1038/s44318-026-00919-5",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13728163/",
  "id": "4pamt7h9qerrg",
  "issns": [
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    "1460-2075"
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  "language": "en",
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      "1312|DNA Repair Mechanisms"
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  "pmcid": "PMC13728163",
  "pmid": "42859192",
  "published": "2026-10-10",
  "publisher": "Springer nature",
  "ris": "https://research.draconitic.com/paper/4pamt7h9qerrg.ris",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.gtp",
      "subfield": "proteomics-metabolomics"
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    {
      "field": "biology",
      "origin": "hal:1.sdv.bc",
      "subfield": "cell-biology"
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  ],
  "title": "Switch from canonical to recombination-dependent DNA synthesis by M-CDK phosphorylation of DNA Polα",
  "title_text": "Switch from canonical to recombination-dependent DNA synthesis by M-CDK phosphorylation of DNA Polα",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:12Z",
  "url": "https://research.draconitic.com/paper/4pamt7h9qerrg",
  "venue": "The EMBO Journal",
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