{
  "abstract": "Background/Objectives: The original GSE283839 study characterized malignant-cell heterogeneity in atypical teratoid/rhabdoid tumor (ATRT). Building on this resource, we investigated the less-characterized glial microenvironment, focusing on astrocyte and microglial transcriptional programs and expression-supported candidate interactions with tumor cells. Methods: We reanalyzed single-nucleus RNA-sequencing data from 12 ATRT tumors using published annotations, program scoring, paired astrocyte–tumor comparisons, and conservative annotation sensitivities. CellChat analyses used 10,000 permutations and within-model Benjamini–Hochberg (BH) correction. An independent cohort comprised eight specimens from four patients. Results: The discovery dataset included 47 annotated astrocytes and 288 annotated microglia. Astrocytes expressed SLC1A2, SLC1A3, and GLUL, with higher glutamate/glutamine-module scores than matched tumor cells in two of three evaluable tumors. This difference persisted in the single tumor evaluable under conservative annotation. Microglia exhibited overlapping inflammatory, immunoregulatory, phagocytic/lipid-processing, and antigen-presentation programs. Across 12 tumors, microglial CD74 expression correlated with the antigen-presentation module score. Candidate interactions included astrocyte-to-tumor NCAM1–FGFR1 and PTN–PTPRZ1 and microglia-to-tumor SPP1-related pairs. Tumor-to-microglia APP–CD74 passed within-model BH correction in 7/10 eligible discovery tumors. External analyses supported tumor-to-myeloid APP–CD74 in at least one myeloid population in each of five evaluable specimens from four patients. Conclusions: This exploratory study highlights astrocyte metabolic expression programs and immune-related microglial programs in ATRT, prioritizing APP–CD74 and additional expression-supported glia–tumor interaction candidates for future spatial and functional investigation.",
  "abstract_shown": true,
  "affiliations": [
    "Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA",
    "Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA",
    "Institute for Pediatric Rare Diseases, Florida State University, Tallahassee, FL 32306, USA"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yu Xue",
      "orcid": "0000-0001-5978-7109"
    },
    {
      "affs": [
        0
      ],
      "name": "Uvindu Thilanka",
      "orcid": "0009-0005-2252-2648"
    },
    {
      "affs": [
        0
      ],
      "name": "Drishty Badhon Sarker",
      "orcid": "0000-0001-6366-3926"
    },
    {
      "affs": [
        0
      ],
      "name": "Md Al Azad"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Qing-Xiang Amy Sang",
      "orcid": "0000-0001-8828-0569"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4ppzmff1ytt41.bib",
  "cited_by": 0,
  "doi": "10.3390/cancers18213455",
  "fulltext_url": null,
  "id": "4ppzmff1ytt41",
  "issns": [
    "2072-6694"
  ],
  "landing_url": "https://www.mdpi.com/2072-6694/18/21/3455",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2716|Glioma Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7223905778",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4ppzmff1ytt41.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:58Z",
      "native_id": "10.3390/cancers18213455",
      "source": "crossref",
      "url": "https://www.mdpi.com/2072-6694/18/21/3455"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2716|Glioma Diagnosis and Treatment",
      "subfield": "human-genetics"
    }
  ],
  "title": "Astrocyte and Microglial Transcriptional Programs in the Atypical Teratoid/Rhabdoid Tumor Microenvironment",
  "title_text": "Astrocyte and Microglial Transcriptional Programs in the Atypical Teratoid/Rhabdoid Tumor Microenvironment",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:58Z",
  "url": "https://research.draconitic.com/paper/4ppzmff1ytt41",
  "venue": "Cancers",
  "versions": []
}