{
  "abstract": "Akabane virus (AKAV) initiates transcription in the cytoplasm via the cap-snatching mechanism. Cytoplasmic host mRNAs act as the primary cap donors, whereas the impact of their subcellular localization on the cap-snatching process remains unclear. Herein, we demonstrate that the AKAV-induced disassembly of polysomes, stress granules (SGs), and processing bodies (PBs) collectively facilitates viral cap-snatching. Mechanistically, AKAV infection activates the PKR-eIF2α signaling axis, leading to the disassembly of polysomes and subsequent translational shutoff. Intriguingly, the viral N protein disassembles SGs and PBs by competing for sequestered RNAs within these granules, whereas mutations of its RNA-binding residues abolish this activity. Moreover, PKR-eIF2α inhibition impairs AKAV replication and cap-snatching efficacy. In contrast, inhibition of SGs via G3BP1 knockdown or chemical agents significantly enhances viral replication and cap-snatching efficiency, indicating that polysomes and SGs disassembly favor caps acquisition. Additionally, N protein-mediated PBs disassembly protects mRNA from degradation, and PBs inhibition enhances cap-snatching. Taken together, AKAV has evolved a sophisticated strategy to reprogram mRNA distribution to create an optimal cellular environment for viral cap-snatching.",
  "abstract_shown": true,
  "affiliations": [
    "Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Beijing University of Agriculture, Beijing, China.",
    "Institute of Animal Inspection and Quarantine, Chinese Academy of Quality and Inspection & Testing, Beijing, China.",
    "School of Agroforestry and Medicine, The Open University of China, Beijing, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zhongyin Liu",
      "orcid": "0009-0002-3119-0946"
    },
    {
      "affs": [
        1
      ],
      "name": "Dongjie Chen",
      "orcid": "0000-0002-0651-6708"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiamin Qin",
      "orcid": "0009-0002-8977-1727"
    },
    {
      "affs": [
        1
      ],
      "name": "Jingjing Wang",
      "orcid": "0000-0002-6658-7735"
    },
    {
      "affs": [
        2
      ],
      "name": "Di Wang",
      "orcid": "0009-0002-9587-9507"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenke Ruan",
      "orcid": "0000-0001-5775-3175"
    },
    {
      "affs": [
        0
      ],
      "name": "Shengkui Xu",
      "orcid": "0009-0003-4698-8392"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4qb5k6sg7gafp.bib",
  "cited_by": 0,
  "doi": "10.1080/21505594.2026.2748149",
  "fulltext_url": "https://europepmc.org/articles/PMC13679326",
  "id": "4qb5k6sg7gafp",
  "issns": [
    "2150-5594",
    "2150-5608"
  ],
  "landing_url": "https://europepmc.org/article/MED/42864310",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|interferon and immune responses"
    ]
  ],
  "openalex": "W7221533310",
  "pdf_url": "https://europepmc.org/articles/PMC13679326?pdf=render",
  "pmcid": "PMC13679326",
  "pmid": "42864310",
  "published": "2026-10-15",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4qb5k6sg7gafp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:46Z",
      "native_id": "MED:42864310",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42864310"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|interferon and immune responses",
      "subfield": "immunology"
    }
  ],
  "title": "Akabane virus reprograms the PKR‑eIF2α‑SGs/PBs axis to fine-tune cap-snatching efficiency.",
  "title_text": "Akabane virus reprograms the PKR‑eIF2α‑SGs/PBs axis to fine-tune cap-snatching efficiency.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:46Z",
  "url": "https://research.draconitic.com/paper/4qb5k6sg7gafp",
  "venue": "Virulence",
  "versions": []
}